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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Wenke Yin Yu Li |
| Copyright Year | 2010 |
| Abstract | Nrf-2(Nuclear factor erythroid 2-related factor 2)/HO-1(Heme Oxygenase-1) signaling pathway plays an important role in the suppression of carcinoma, The study aims to investigate the effect of Curcumin on the expression of HO-1 and Nrf-2, which will provide a therapeutic intervention for treatment of carcinoma. SHSY5Y cells were treated with Curcumin at 0, 1.25, 5.0, 20.0 µM for the time course assay. RT-PCR and Western Blot assays were carried out to detect mRNA and protein expression of Nrf-2 and HO-1, we also transfect Nrf-2 siRNA after SHSY5Y cells were treated with Curcumin at different dosage at 24 hr. RT-PCR and Western blot results indicated that the expression of HO-1 mRNA and protein significantly increased, and the changes were in a dose- and time-dependence manner, so did the Nrf-2. Moreover, the expression of HO-1 protein in the Nrf-2 siRNA transfected cells were sharply decreased after treated with Curcumin at different dosage at 24 hr. HO-1 is a potential target for treating carcinoma. The mechanism of Curcumin playing its role of suppressing carcinoma is via activiting Nrf-2/HO-1 pathway. The results will provide a new method for treatment of carcinoma with Curcumin. |
| Starting Page | 1 |
| Ending Page | 4 |
| File Size | 344361 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781424447121 |
| ISSN | 21517622 |
| DOI | 10.1109/ICBBE.2010.5516462 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2010-06-18 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Proteins Pathology Image analysis Neuroscience Ethanol RNA Cells (biology) Biomembranes Biochemistry Extracellular |
| Content Type | Text |
| Resource Type | Article |
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