NDLI: Repair of Deep Burn Skin Defects with Cultured Epidermal Stem Cells and Acellular Amniotic Membranes

Content Provider	IEEE Xplore Digital Library
Author	Dewu Liu Honglan Xiong Yuangui Mao Peixin Huang Jianping Chen Wei Lan
Copyright Year	2010
Abstract	Tissue engineered skin constructed by cultured seed cells provides a new way to accelerate skin wound healing and improve the quality of tissue repair. Conventional models with dermal scaffold material based on collagen hydrogels, however, have poor stability and unsatisfactory longevity. Here, we describe an improved acellular amniotic membrane as a scafford with epidermal stem cells for tissue engineering skins and observe their clinical effect on deep burn wounds. The human epidermal stem cells was isolated from the skin samples by trypsin digesting method and purified by collagen adhering method, and then seeded on the acellular amniotic membrane to form the engineered skin. The biological attachment and growth of cultured epidermal stem cells were observed. Twelve cases of deep burn wounds were applied with the engineered skin. The results showed that the epidermal stem cells adhered to the surface of acellular amniotic membrane quickly after being seeded and exhibited a high colony formation efficiency. The wounds treated with engineered skin healed rapidly with good clinical "take". The average time of wound closure in engineered skin group was shortened than that of control group. There were no obvious evidence of immunological rejection and inflammatory reaction during the observation. This indicated that the acellular amniotic membrane with good histocompatibility may be a suitable scaffold with epidermal stem cells in the construction of tissue engineering skin to repair deep burn wounds.
Starting Page	1
Ending Page	3
File Size	239796
Page Count	3
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5517500
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Tissue engineering Stem cells Cells (biology) Wounds Dermis Epidermis Skin Biomembranes Acceleration Biological materials
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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