NDLI: Differentially Expressed cDNAs in Cold-Stressed SSH Library of Ceratoides lanata

Content Provider	IEEE Xplore Digital Library
Author	Yan-Ling Zeng Dang-Quan Zhang Fu-Huan Ming Zhi-Dan Song Xin Han Ping Jiang
Copyright Year	2010
Abstract	As a high stress resistance psammophyte with the characteristic of sand fixation and winterfat, Ceratoides lanata had been successfully introduced into many countries in recent years. The leaves and twigs of Ceratoides lanata in room temperature were used as the Driver and those treated by cold stress were used as Tester. Tester and Driver ds cDNA were prepared from their high quality mRNA which were purified from their total RNA. Tester and Driver cDNA were separately digested to obtain shorter, blunt-ended molecules by RasI. Then two Tester populations are created with different adaptors, while Driver cDNA has no adaptors. Differentially expressed genes were equalized and enriched by two round subtractive hybridizations using excess Driver population as compared with Tester population. The differentially expressed cDNAs were exponentially amplified by first round suppression PCR using the diluted hybridization product as template, and then the secondary PCR was performed using the first PCR product as template by nested primers to finally enrich the differentially expressed cDNAs, which consists of the SSH library of Ceratoides lanata. These cDNAs were inserted into vectors and 362 cDNA clones were obtained. The sequencing results showed that some cDNAs of cold-stressed Ceratoides lanata SSH library have relatively high homology with known stress resistance-related genes or proteins, and other cDNAs are new genes which are firstly reported here. Our result lays a foundation for the cDNA cloning of valuable genes including antifreeze genes, heat-resistant genes, drought-tolerant genes, alkali-salt-tolerant genes from Ceratoides lanata, and their transgenic application
Starting Page	1
Ending Page	3
File Size	226751
Page Count	3
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5516977
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Temperature Cloning Forestry Anti-freeze Libraries Page description languages Genetic engineering Stress Injuries Testing
Content Type	Text
Resource Type	Article

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