NDLI: Anticancer Effect of Gambogic Acid in Gastric Cancer Line SGC-7901

Content Provider	IEEE Xplore Digital Library
Author	Ning Zhong Dong Jiang Shi-Ying Zheng
Copyright Year	2010
Abstract	We investigate the anticancer effect of a traditional Chinese medicine gambogic acid (GA) in human gastric cancer line SGC-7901 and further study the mechanism of apoptosis induction of GA.Low differential human gastric cancer line SGC-7901 were treated with GA at different doses and different times, the inhibitory rates were detected by MTT assay. Apoptosis induced by GA in SGC-7901 cells was observed by Annexin-V/PI doubling staining flow cytometry assay. And T/C (%) was chosen to detect the inhibition of GA on human gastric adenocar-cinoma SGC-7901 nude mice xenografts. Apoptosis on nude mice xenografts was observed by Annexin-V/PI doubling staining flow cytometry assay and DNA fragmentation assay. To further determine the molecular mechanism of apoptosis induced by GA, the changes on the expression of bcl-2 and bax genes were detected by RT-PCR.After incubation with GA, low differential human gastric cancer line SGC-7901 was dramatically inhibited in a dose-dependent manner. After these cells were exposed to GA for 24, 48 and 72 h, the IC50 value was 1.02±±0.05, 1.41±±0.20 and 1.14±± 0.19 µmol/L, respectively. Apoptosis in SGC-7901 cells induced by GA was observed by Annexin-V/PI doubling staining flow cytome-try assay. The apoptotic population of SGC-7901 cells was about 12.96% and 24.58%, respectively, when cells were incuba-ted with 1.2 µmol/L GA for 48 and 72 h. T/C (%) of human gastric carcinoma adeno-carcinoma SGC-7901 nude mice xenografts was 44.3, when the nude mice were treated with GA (8 mg/kg). Meanwhile, apoptosis induced by GA was observed in human gastric carcinoma adenocarcinoma SGC-7901 nude mice xenografts. The increase of bax gene and the decrease of bc1-2 gene expressions were found by RT-PCR.The inhibition of GA on human gastric cancer line SGC-7901 was confirmed. This effect connects with the inducing apoptosis in SGC-7901 cells and the molecular mechanism might be related to the reduction of expression of apoptosis-regulated gene bcl-2, and the improvement of the expression of apoptosis-regulated gene bax. The result was also confirmed in vivo.
Starting Page	1
Ending Page	6
File Size	340380
Page Count	6
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5516997
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	In vivo Hospitals Animals Oncological surgery Humans Cells (biology) Mice Performance analysis Neoplasms Cancer
Content Type	Text
Resource Type	Article

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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