NDLI: Influence of Trehalose Accumulation on Response to Freeze Stress in Baker's Yeast

Content Provider	IEEE Xplore Digital Library
Author	Cui-ying Zhang Dong-guang Xiao Ye Lv
Copyright Year	2010
Abstract	Accumulation of trehalose is suggested to be a critical determinant in improving the stress tolerance of the yeast Saccharomyces cerevisiae. To test the influence of trehalose accumulation on response to freeze stress, we constructed all single and double gene disruption mutants of the trehalase genes ATH1, NTH1 and NTH2 and examined the changes of their trehalose accumulations and their freeze tolerances. Under the condition of freeze stress, three mutants, Δnth1, Δnth2Δnth1, and Δath1Δnth1, all of which carry the NTH1 gene disruption, showed increased trehalose accumulation as compared to the parent strain and other mutants. Moreover, compared with the parent strain BY-6, the NTH1 disruption mutants possessed higher cell survival ratios and greater relative leavening abilities. The results indicated that NTH1 gene is important for trehalose degradation under conditions of freeze stress, thus closely correlated with freeze tolerance of baker's yeast.
Starting Page	1
Ending Page	4
File Size	293263
Page Count	4
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5515246
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fungi Degradation Biotechnology Microorganisms DNA Production Capacitive sensors Biochemistry Stress Immune system
Content Type	Text
Resource Type	Article

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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