NDLI: Establishment and Identification of Cold-Stressed Normalized Full-Length cDNA Library of Ceratoides lanata

Content Provider	IEEE Xplore Digital Library
Author	Dang-Quan Zhang Fu-Huan Ming Quan-Dong Zhu Zhi-Dan Song Xin Han Ping Jiang
Copyright Year	2010
Abstract	Ceratoides lanata is the native sand-fixing plant in deserts of America Continent. As an even-green undershrub with extremely strong antifreeze capability, Ceratoides lanata had been considered as the winterfat and introduced into many countries including China. To explore the mechanism of cold resistance and to clone genes directly involving the strong antifreeze capability, a normalized cold-stressed full-length cDNA library was constructed from the cold-treated leaves of Ceratoides lanata by the method of DSN (duplex-specific nuclease)-based normalization and SMART (switching mechanism at 5' end of RNA transcript) full-length cDNA construction. The quality check showed that the titer of un-amplified cDNA library was about 1.4×106 cfu mL−1 and the average size of cDNA inserts was about 1 150 bp with a recombination rate of 100%. The result of sequencing 187 clones selected randomly revealed that the redundancy of the cold-stressed normalized full-length cDNA library is only 0.59%, and the ratio of full length is 46.3%. Among 187 cDNAs, 24 have homology with known genes or proteins, while the other 163 have no identity, suggesting that most cDNAs are new cold-involved genes from Ceratoides lanata. The analytic result showed that the cold-stressed normalized full-length cDNA library of Ceratoides lanata a was established successfully, and this will benefit the further study on the molecular mechanism of strong antifreeze capability and cloning of cold-resistant genes
Starting Page	1
Ending Page	3
File Size	367482
Page Count	3
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5517719
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Cloning Forestry Anti-freeze Libraries Page description languages Genetic engineering Ice Continents Injuries Stress
Content Type	Text
Resource Type	Article

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