NDLI: Dynamics of Nitrobacteria Community in Biological Contact Oxidation Process by DGGE and FISH

Content Provider	IEEE Xplore Digital Library
Author	Shuying He Jing Chen Jixiang Li Yatong Xu Haizhen Yang
Copyright Year	2010
Abstract	The performance and nitrobacter population dynamics of a pilot-scale biological contact oxidation process which was used to purify a landscape river, were evaluated during the biofilm forming period over 45 days. Ammonia oxidizing bacteria (AOB) community structure and composition of nitrobacteria in the system were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and fluorescence in situ hybridization (FISH) techniques. The results showed that 30% average removal efficiency of NH4+-N and 15% average removal efficiency of chemical oxygen demanded (CODMn) were achieved. DGGE analysis represented a dynamic population with gradually evolved dominant species. The number of superior bands increased with the development of biofilm, and Data from 16S rRNA sequencing demonstrated genera Nitrosomonas was the dominant AOB in this system. FISH results suggested that AOB and nitrite-oxidizing bacteria (NOB) accounted for high up to 9.9% and 3.8% of the total bacteria, respectively, and Nitrospira was the dominant members of NOB in the biofilm. The general nitrobacteria population coupled with the performance of biological contact oxidation process indicated that the dynamics of the community structure of nitrobacteria was important indicator to evaluate the effect of the biofilter system.
Starting Page	1
Ending Page	4
File Size	1308776
Page Count	4
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5515470
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Materials testing Microorganisms Fluorescence Educational institutions Oxidation Biology Rivers Phylogeny Marine animals Environmental economics
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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