NDLI: Molecular and Phenotypic Characterization of Transgenic Tobacco Expressing the Arabidopsis IRT1 Gene

Content Provider	IEEE Xplore Digital Library
Author	Li Liya Cai Liya Jia Kai Zhang Meng Guo Changhong
Copyright Year	2010
Abstract	In order to enhance the iron accumulation in plants, the primers were designed based on the Arabidopsis thaliana IRT1 sequence in GenBank. The total RNA of Arabidopsis thaliana roots was extracted after iron deficiency treatment for 24h. The cDNA of IRT1 gene was cloned by RT-PCR and then was inserted into the pBluescript II SK+ vector. After confirmed by endonuclease digestion assay and gene sequencing, the IRT1 gene was subcloned into the Sac I and BamH I targeting sites of the vector pBI121, and the plant expression vector pBI121IRT1 was constructed. The pBI121IRT1 vector was transformed into the wild type tobacco by Agrobacterium mediated method. Seventy-six resistant shoots were obtained by Kanamycin selection. 21 were identified by PCR and PCR-Southern detection. Five transformants were selected to perform the physiological assay respectively. The results showed the leaf chlorophyll contents were higher in the transformants than in the control, while the leaf MDA contents were lower in the transformants. It indicated that the IRT1 gene enhanced the iron uptake in the transformants and improved the transformants′ resistance to iron deficiency.
Starting Page	1
Ending Page	4
File Size	481980
Page Count	4
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5516422
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fungi Proteins Sequences Plants (biology) RNA Crops Soil Iron Electronic mail Immune system
Content Type	Text
Resource Type	Article

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