NDLI: An alpha-Amylase Biosensor with 1,1-Dimethyl-3-(2-Amino-1-Hydroxyethyl) Ferrocene as an Electron Transfer Mediator

Content Provider	IEEE Xplore Digital Library
Author	Jun Zheng Guoming Xie Changtao Chen
Copyright Year	2010
Abstract	A new disposable α-amylase biosensor has been developed based on the encapsulation of both the glucose oxidase (GOD) and the α-glucosidase in the composite film of the sol-gel/Nafion.1,1-dimethyl-3-(2-amino-1-hydroxyethyl) ferrocene (DMAHFc) as an electron transferring mediator was immobilized on the screen-printed electrode surface by Nafion. Under optimal conditions this biosensor was able to detect α-amylase activity in the linear range of 15-980 IU L-1 (R2 = 0.9523), which was wider than some of other α-amylase biosensors using the flow-injection-type assay and some of other biosensors using the gelatin membrane. Three components DMAHFc, sol-gel and Nafion not only enhanced the electron transfer, but also improved the sensitivity and long-term stability of the biosensor. The limit of detection of 1.2 IU L-1 was obtained, in comparison to detection limits of 50 IU L-1 obtained by some of other α-amylase biosensors using the flow-injection-type assay and 17 IU L-1 obtained by some of other biosensors using the gelatin membrane. The activity of salivary α-amylase can be detected by the biosensor with a fast response, high sensitivity, good reproducibility, and long-term stability. So, it is possible to construct a new method to identifies people's mental stress conditions conveniently.
Starting Page	1
Ending Page	5
File Size	328325
Page Count	5
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5516032
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrodes Encapsulation Temperature Stability Biochemical analysis Ink Human factors Biosensors Sugar Electrons
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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