NDLI: A New Method to Evaluate Risk Alleviation of PAHs Contaminated Sites

Content Provider	IEEE Xplore Digital Library
Author	Zhenyi Zhang Inoue, C. Guanghe Li
Copyright Year	2010
Abstract	Environmental risk is ascribed to contamination level as well as the potential decontamination capability of the contaminated sites. In some cases, natural attenuation by biodegradation may dominate self cleanup as microorganisms always try to survive, which reduces risk to human health. Conventional assessment is based on sequential monitoring of concentrations, which requires a large number of samples and long time. In addition, some organic compounds like polycyclic aromatic hydrocarbons(PAHs) are hard to be accurately measured due to their hydrophobic properties. The paper presented a new method to evaluate risk alleviation of PAHs contaminated sites. Instead of conventional method, the new method proposed DNA copies/cell quantity ratio as an indicator to evaluate microbial activity which indirectly reflects the trend of risk alleviation. The concept was theoretically elaborated and verified by lab experiments. In this study, Mycobacterium vanbaalenii PYR-1, a typical PAHs degrading bacterium, was introduced and phenanthrene was used as the sole PAHs substrate. The DNA copies and cell quantity were determined by real time polymerase chain reaction (PCR) and colony forming units (CFU) method respectively. Conventional method was also carried out as controls. The results implied the new method may be a fast evaluation of risk alleviation and potentially supportive for engineering applications.
Starting Page	1
Ending Page	4
File Size	319120
Page Count	4
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5518055
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Biodegradation Microorganisms Decontamination DNA Potential well Humans Attenuation Contamination Monitoring Organic compounds
Content Type	Text
Resource Type	Article

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