NDLI: An Unusual Pectate Lyase with Noticeable Effects on the Chinese Medicinal Herb Extract: Cloning and Characterization

Content Provider	IEEE Xplore Digital Library
Author	Jing Xiao Fu-Ping Lu Yu Li Jun-Xun Li
Copyright Year	2010
Abstract	Pectate lyase obtained the wide-spread application in the Chinese traditional medicine extraction because it can remarkably reduce impurity content and viscosity of the plant materials. However the low enzyme activity and monoicous species hindered the application of enzymatic method to the extraction process of the herbal medicine. In this paper, we constructed a engineering strain which can overexpressed pectate lyase. The pectate lyase activity of gene engineering bacteria was even 100 times of the wild strain. The optimal temperature and pH of recombinant enzyme were 60℃ and pH10.5.When the recombinant enzyme was used in the extraction process of flax seeds, the viscosity of the raw materials could be reduced by more than 45% and linseed oil content could be increased by 12%.The usage of pectate lyase in the extraction process showed to be a feasible way to promote the product development process of flax seeds or other medicinal herbs.
Starting Page	1
Ending Page	4
File Size	246407
Page Count	4
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5516496
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Viscosity Microorganisms Temperature Impurities Cloning Biochemistry Capacitive sensors Raw materials Product development Petroleum
Content Type	Text
Resource Type	Article

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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