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Content Provider | IEEE Xplore Digital Library |
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Author | Hui Yuan Xiao Kun Li |
Copyright Year | 2010 |
Abstract | Non-mitogenic form of recombinant Human acidic fibroblast growth factor (nrhaFGF) retained the cardio and neuro-protective characters of the wild-type aFGF while decreased the potential side effects like mitogenesis activity. In this paper, nrhaFGF was successfully expressed in Escherichia coli and yielded about 5% of the total soluble protein in the 10L fermentation tank. the succeedent chromatography, cation exchange and heparin affinity gained the purity 55.0% and 95.6% sepecifically, after desalting with Sephadex G 25, aggregation was found increased 2-4% after the lyophilization, and Sephadex G75 completely eliminated the aggregates while untrafiltration with cellulose triacetate membrane left 2% aggregates in the samples. The chromatography method and the the aggregation removal method with size exclusion chromatography were easily scaled up and spade the work for the later clinical trials. |
Starting Page | 1 |
Ending Page | 5 |
File Size | 369959 |
Page Count | 5 |
File Format | |
ISBN | 9781424447121 |
ISSN | 21517622 |
DOI | 10.1109/ICBBE.2010.5518200 |
Language | English |
Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Publisher Date | 2010-06-18 |
Publisher Place | China |
Access Restriction | Subscribed |
Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Subject Keyword | Proteins Purification Aggregates Laboratories Humans Fibroblasts Clinical trials Biomembranes Cardiology Chemical technology |
Content Type | Text |
Resource Type | Article |
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