NDLI: Structural Characterization of the DNA Adducts Induced by 1,3-Bis-(2-Chloroethyl)-1-Nitrosourea Using Electrospray Ionization Tandem Mass Spectrometry

Content Provider	IEEE Xplore Digital Library
Author	Bao-Qing Bai Li-Jiao Zhao Ru-Gang Zhong
Copyright Year	2010
Abstract	Bifunctional alkylating agent 1,3-Bis-(2-chloroethyl)-1-nitrosourea (BCNU) is a significant anticancer drug in the clinical treatment of human malignancies. A serious complication of treatment with BCNU is the increased risk of a secondary leukaemia in long-term survivors because not all alkylating agent interactions with DNA result in cell death. In the present work, high-performance liquid chromatography (HPLC) was combined with electrospray ionization tandem mass spectrometry (ESI-MS/MS) to develop a sensitive and selective method for the measurement of DNA adducts induced by BCNU. Double strand calf thymus DNA was treated with BCNU and digested enzymatically down to the nucleoside level. The hydrolysates were analyzed by HPLC separation followed by electrospray ionization tandem mass spectrometry. The main alkylated products, N7-(2-hydroxyethyl)deoxyguanosine (N7-HOEtdG), N7-(2-chloroethyl)deoxyguanosine (N7-ClEtdG) and 1-[N3-deoxycytidyl]-2-[N1-deoxyguanosyl]ethane (dG-dC) were qualitatively detected and quantitatively characterized by selected reaction monitoring (SRM) mode.
Starting Page	1
Ending Page	4
File Size	409757
Page Count	4
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5516358
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Water Solvents Temperature Ethanol Ionization DNA Humans Mass spectroscopy Educational institutions Monitoring
Content Type	Text
Resource Type	Article

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