NDLI: cDNA, Genomic Sequence Cloning and Sequences Analysis of the fau Gene from the Giant Panda (Ailuropoda melanoleuca)

Content Provider	IEEE Xplore Digital Library
Author	Guang-fu Wu Yi-ling Hou Wan-ru Hou Yan Song Tian Zhang
Copyright Year	2010
Abstract	fau gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein FUBI at the N-terminus and ribosomal protein S30 at the C-terminus. Here we have cloned the cDNA and the genomic sequence of fau gene from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and Touchdown-PCR, respectively. The length of of cDNA fragment cloned contains an open reading frame of 414 bp encoding 133 amino acids which estimated molecular weight of the fau is 14.4277 kDa with an isoelectric point (pI) of 10.17 and the length of the genomic sequence is 1143 bp, containing four exons and three introns. Alignment analysis revealed that the nucleotide sequence and the deduced protein sequence are highly conserved to other five species studied, including Mus musculus, Rattus norvegicus, Bos taurus, Sus scrofa, and Homo sapiens. The homologies for nucleotide sequences of giant panda fau to that of these species are 89.80%, 89.55%, 91.29%, 92.79%, and 91.79%, respectively, and the homologies for amino acid sequences are 96.99%, 97.74%, 96.99%, 98.50%, and 96.99%, respectively. Topology prediction showed that there are one cAMP- and cGMP-dependent protein kinase phosphorylation site, two Protein kinase C phosphorylation sites, four Casein kinase II phosphorylation sites, and one Amidation site in the fau protein of the giant panda.
Starting Page	1
Ending Page	6
File Size	577842
Page Count	6
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5517214
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Sequences Genomics Cloning Humans Amino acids Mice Encoding Bioinformatics Tumors
Content Type	Text
Resource Type	Article

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