NDLI: Identification and Sequence Analysis of the Duck Plague Virus UL46 Gene

Content Provider	IEEE Xplore Digital Library
Author	Liting Lu Anchun Cheng Mingshu Wang Dekang Zhu Xiaoyue Chen Renyong Jia Qihui Luo Yi Wang Zhiwen Xu Zhengli Chen
Copyright Year	2010
Abstract	The DPV UL46 gene( GenBank accession No. EU195108) isolated by our laboratory was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmid pMD18-T-UL46 was identified by PCR, restriction enzymes (BamHⅠ+XhoⅠ), sequencing and nucleic acid dot-hybridization. Then the gene was analyzed by bioinformatics tools. The results showed that a 2220bp complete open reading frame of 739 amino acids with Mr 81.8 kDa and PI 9.00 was discovered and it shared low homology with 24 strains other herpesvirus UL46 gene. The phylogenetic tree of the amino acids sequences indicated that the DPV should be placed into a single cluster within the subfamily Alphaherpesvirinae. The DPV UL46 had 10 transcription promo-ter sequences and a functional domains between 17-470aa. Codon bias analysis showed that the UL46 gene was biased in some codons, especially those codons encoding amino acids Trp, Met, His, Lys and Glu. The 35 epitopes covered the whole polypeptide chains of VP11/12, where hydrophilicity regions took the most parts. It had better hydrophilicity, flexible regions and higher antigen indexs. The DPV UL46 gene was predicted to be situated outside of membrane, locating throughout the cytoplasm with concentrations in the perinuclear region. It had 72 phospho-rylation sites and 2 N-Glycosylation potential sites but signal peptide. The protein had many flexible regions, α-helices and β-strands, but only a few of random coils. The tertiary structure had not been gained by the way of homology modelling of molecular confirmation. These results provided elementary data to research the biological function of this gene.
Starting Page	1
Ending Page	8
File Size	497715
Page Count	8
File Format	PDF
ISBN	9781424447121
ISSN	21517622
DOI	10.1109/ICBBE.2010.5516178
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-06-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Sequences Peptides Laboratories Amino acids Biochemistry Capacitive sensors Encoding Biomembranes Phylogeny Bioinformatics
Content Type	Text
Resource Type	Article

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