NDLI: Fabrication of microfluidic system for the assessment of cell migration on 3D micropatterned substrates

Content Provider	IEEE Xplore Digital Library
Author	Eun-Joong Lee Chang-Mo Hwang Dong-Hyun Baek Sang-Hoon Lee
Copyright Year	2009
Description	Author affiliation: School of Electrical Engineering and Department of Biomedical Engineering, College of Health Science, Korea University, Jeongneung-dong, Seongbuk-gu, Seoul 136-703, Republic of Korea (Dong-Hyun Baek) \|\| Department of Biomedical Engineering, College of Health Science, Korea University, Jeongneung-dong, Seongbuk-gu, Seoul 136-703, Republic of Korea (Eun-Joong Lee; Sang-Hoon Lee) \|\| Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, MA 02139 USA (Chang-Mo Hwang)
Abstract	Cell migration and proliferation are major process in wound healing, cancer metastasis and organogenesis during development. Many cells are related to recovery process of wound. Especially, fibroblasts act an important role in wound healing. Various cytokines such as platelet derived growth factor (PDGF) can induce fibroblast migration and widely studied to investigate the cell response under controlled cytokine microenvironments during wound healing. In real tissue healing process, cell microenvironments change with tissue types and anatomical characteristics of organs. With microfluidic system, we tried to mimic the natural microenvironment of wound healing, with gradient of PDGF, a fibroblast migration inducing cytokine, and patterned substrate with different orientation to PDGF gradient. Fibroblasts cultured in PDGF gradient micro fluidic chip showed cell migration under various micro environmental gradient conditions. Cells were cultured under PDGF gradient condition and different substrate pattern. Mouse fibroblast L929 cells were cultured in the microfluidic gradient. The results showed that most cells migrated along the substrate topological patterns under high concentration of PDGF. We developed long range sustaining micro fluidic channel and could analyze cell migration along the gradient of PDGF. Also, the cell migration on patterned extracellular environment shows that cells migrate along the extracellular 3D pattern rather than directly along the cytokine gradient when the pattern height is less than 1 μm. In this study, we could demonstrate that the extracellular pattern is more dominant to cell migration in combination with cytokine gradient in the wounded tissue when the environmental cues are 20 μm.
Starting Page	6034
Ending Page	6037
File Size	1096126
Page Count	4
File Format	PDF
ISBN	9781424432967
ISSN	1557170X
DOI	10.1109/IEMBS.2009.5333169
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-09-03
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fabrication Microfluidics Wounds Fibroblasts Cells (biology) Extracellular Fluidic microsystems Cancer Metastasis Mice
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Evaluation of Silicon and Polymer substrates for fabrication of integrated microfluidic microsystems for DNA extraction and amplification

PCB/Polymer based micro-fluidic system for NMR Spectroscopy for nanoliters sample volume

Microfluidic device with dual mechanical cues for cell migration investigation

Separation and capture of circulating tumor cells from whole blood using a bypass integrated microfluidic trap array

3D Fabrication of Biological Machines

Implantable micropump technologies for murine intracochlear infusions

Biological microdevice with fluidic acoustic streaming for measuring uric acid in human saliva

Geometry-Dependent Behavior of Fibroblast Cells in Three-Dimensional Silicon Microstructures

Nanograting structure promotes lamellipodia-based cell collective migration and wound healing

Fabrication of microfluidic system for the assessment of cell migration on 3D micropatterned substrates

Similar Documents

Evaluation of Silicon and Polymer substrates for fabrication of integrated microfluidic microsystems for DNA extraction and amplification

PCB/Polymer based micro-fluidic system for NMR Spectroscopy for nanoliters sample volume

Microfluidic device with dual mechanical cues for cell migration investigation

Separation and capture of circulating tumor cells from whole blood using a bypass integrated microfluidic trap array

3D Fabrication of Biological Machines

Implantable micropump technologies for murine intracochlear infusions

Biological microdevice with fluidic acoustic streaming for measuring uric acid in human saliva

Geometry-Dependent Behavior of Fibroblast Cells in Three-Dimensional Silicon Microstructures

Nanograting structure promotes lamellipodia-based cell collective migration and wound healing

Fabrication of microfluidic system for the assessment of cell migration on 3D micropatterned substrates