NDLI: Device for the control of oxygen concentration in multiwell cell culture plates

Content Provider	IEEE Xplore Digital Library
Author	Oppegard, S.C. Eddington, D.T.
Copyright Year	2009
Description	Author affiliation: University of Illinois at Chicago, Chicago, IL 60607 USA (Oppegard, S.C.) \|\| Bioengineering Department, University of Illinois at Chicago, Chicago, IL 60607 USA (Eddington, D.T.)
Abstract	Oxygen is a key modulator of many cellular pathways but current devices permitting in vitro oxygen modulation fail to meet the needs of many researchers. In this study, a microfabricated insert for multiwell formats has been developed to control the gas concentration of each well independent of the global incubator's condition. The platform consists of a polydimethylsiloxane (PDMS) insert that nests into a standard multiwell plate and serves as a passive network with a gas permeable membrane aimed to deliver gas to adherent cell cultures. Preliminary data demonstrate that the insert is effective in controlling the oxygen concentration at the cell surface inside a well with equilibration times in minutes rather than hours for conventional technologies. A wide variety of oxygen profiles can be attained based on the device design, such as the cyclic profile achieved in this study, and even gradients in local oxygen concentration to mimic those found in vivo for more biomimetic cellular models.
Starting Page	2097
Ending Page	2100
File Size	744253
Page Count	4
File Format	PDF
ISBN	9781424432967
ISSN	1557170X
DOI	10.1109/IEMBS.2009.5332491
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-09-03
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Oxygen Biomembranes Microchannel Cells (biology) In vivo In vitro Biological system modeling Passive networks Biochemistry Organisms
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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