NDLI: Conduction analysis in mixed cardiomyocytes-fibroblasts cultures using microelectrode arrays

Content Provider	IEEE Xplore Digital Library
Author	Roy, S. Chen, M.Q. Kovacs, G.T.A. Giovangrandi, L.
Copyright Year	2009
Description	Author affiliation: Department of Bioengineering, Stanford University, Stanford, CA 94305, USA (Chen, M.Q.) \|\| Departments of Electrical Engineering & Medicine, Stanford University, Stanford, CA 94305, USA (Kovacs, G.T.A.) \|\| Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA (Roy, S.; Giovangrandi, L.)
Abstract	Models for cardiac arrhythmia currently exist primarily in in-vivo and computer simulation form. Towards the development of such a model in-vitro, a better understanding of electrical conduction in heterogeneous cultures is required. Increasing ratios of cardiomyocytes and fibroblasts were cultured on 500 × 500 µm arrays of 36 microelectrodes to study the emergence and properties of action potential propagation in mixed cultures. A minimum ratio of 70% cardiomyocytes to 30% fibroblasts was found to be necessary for detection of electrical activity. However, the establishment of a continuous, homogeneous depolarization wave across the culture required a higher proportion of cardiomyocytes; even a 90:10 ratio was unable to consistently produce a unidirectional, uniform depolarization wave as is seen in controls. This model underlines the importance and sensitivity of tissue homogeneity in supporting electrical conduction, and is especially relevant to studies of arrhythmia (reentry) and stem cell grafts.
Starting Page	4250
Ending Page	4253
File Size	1657197
Page Count	4
File Format	PDF
ISBN	9781424432967
ISSN	1557170X
DOI	10.1109/IEMBS.2009.5333605
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-09-03
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Cardiology Microelectrodes Heart In vitro Fibroblasts Stem cells USA Councils Computer simulation Proportional control Pacemakers
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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