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Full-Length cDNA Libraries: Reagents for Functional Studies of the Nervous System
| Content Provider | Scilit |
|---|---|
| Author | Hida, Munetomo Suzuki, Yutaka Sugano, Sumio |
| Copyright Year | 2001 |
| Description | The genomic revolution manifested by completion of human and other mammalian genome sequences and the ready public access to complementary DNA (cDNA) libraries, sequences, and clones will impact greatly on conceptual approaches and experimental strategies for our understanding of nervous system function and complex behaviors. While the development of systematic cDNA resources utilizing the expressed sequence tag (EST) strategy has led to discovery of EST clones and generation of nonredundant collection of genes found in the nervous system, cDNA libraries enriched in full-length cDNA clones are often required for assessing gene functions in their full biological context. A “full-length cDNA library” contains a significantly higher percentage of full-length DNA clones than a cDNA library constructed by standard $methods.^{ 1 }$ The full-length cDNA serves as effective material for functional analysis of a gene (Figure 9.1), e.g., recombinant protein $synthesis,^{ 2-3 }$ protein $characterization^{ 4–7 }$ of newly isolated genes, generation of knockout $mice,^{ 8,9 }$ and expression profiling by microarray $analysis.^{ 10 }$ Book Name: Methods in Genomic Neuroscience |
| Related Links | https://content.taylorfrancis.com/books/download?dac=C2010-0-33430-9&isbn=9780429126192&doi=10.1201/9781420038477-13&format=pdf |
| Ending Page | 228 |
| Page Count | 22 |
| Starting Page | 207 |
| DOI | 10.1201/9781420038477-13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2001-09-26 |
| Access Restriction | Open |
| Subject Keyword | Book Name: Methods in Genomic Neuroscience Cell Biology Length Cdna Library Full Length Cdna Libraries |
| Content Type | Text |
| Resource Type | Chapter |
