NDLI: Expression of telomerase reverse transcriptase gene in human epidermal stem cells and its significance

Content Provider	IEEE Xplore Digital Library
Author	Dewu Liu Lianqun Wang Pu Ning Wei Lan Peixing Huang
Copyright Year	2010
Description	Author affiliation: Institute of Burn, First Affiliated Hospital of Nanchang University, Jiangxi Province, China (Dewu Liu; Lianqun Wang; Pu Ning; Wei Lan; Peixing Huang)
Abstract	It is well known that the telomerase is present at high levels in embryonic tissues, which plays an important role in replication and proliferation capacity of the stem cells. But the telomerase activity in cultured epidermal stem cells derived from human skins is not fully understood. In this chapter, we focus on the isolation, culture and identification of the epidermal stem cells derived from human fetal skins, and investigating the mRNA and protein expression of telomerase reverse transcriptase in cultured epidermal stem cells. The telomerase activity and the mRNA and protein expression of telomerase reverse transcriptase were determined by using TRAP-ELISA, western blot, immunocytochemical stain and RT-PCR techniques, respectively. The results showed that the cultured epidermal stem cells revealed colony growth. The telomerase activity and the mRNA and protein expression of telomerase reverse transcriptase were positive and in low level in cultured epidermal stem cells. It indicated that the expression of telomerase activity in human epidermal stem cells seems to be directly associated with their proliferation capacity and may play a critical role in overcoming growth limitations of seed cells in tissue engineering skins.
Starting Page	1673
Ending Page	1676
File Size	310580
Page Count	4
File Format	PDF
ISBN	9781424464951
e-ISBN	9781424464982
DOI	10.1109/BMEI.2010.5639622
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-10-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins tissue engineering wound healing Humans Stem cells Wounds skin Epidermis epidermal stem cell telomerase reverse transcriptase Immune system
Content Type	Text
Resource Type	Article

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