NDLI: Capillary fractionation of HPLC substrates by a microfluidic droplet generator for high throughput analysis

Content Provider	IEEE Xplore Digital Library
Author	Hamed, S. Shay, B. Basu, A.S.
Copyright Year	2011
Description	Author affiliation: Biomedical Mass Spectrometry Facility in the Department of Pharmacology at the University of Michigan Ann Arbor (Shay, B.) \|\| Department of Biomedical Engineering, Wayne State University, Detroit MI (Hamed, S.) \|\| Department of Electrical and Computer Engineering and the department of Biomedical Engineering at Wayne State University (Basu, A.S.)
Abstract	Biochemical samples are complex mixtures containing 1000's of components which often must be fractionated prior to analysis. Conventional fraction collectors, which can only accommodate 10's of fractions, are not well suited for high throughput analysis. This paper describes microfractionation in droplets (μFD), a scalable microfluidic technique for generating thousands of fractions. A drop generator, placed downstream from a high performance liquid chromatography (HPLC) column, encapsulates the separated components into a serial array of monodisperse droplets. The droplets can be stored in a capillary or immediately used in subsequent assays. Using μFD, a mixture of 3 dyes separated in a C18 column was fractionated into 2,160 droplets in <6 min. The volume and frequency of the droplet fractions are governed by the capillary number (Ca), which depends on the viscosity of the carrier fluid, flow rate, and interfacial tension. With HPLC-compatible flow rates of 0.38–0.7 mL/min, in a 1.5 mm Teflon capillary, fractions contain volumes of 1–6 μL and are generated at 2–10 drops/s. Droplet fractions can be mixed with a subsequent reagent using a downstream tee junction. In theory, μFD can be coupled to a wide variety of separation processes, enabling high throughput fractionation and screening of complex mixtures in μL to sub-nL volumes.
Starting Page	8396
Ending Page	8399
File Size	1158024
Page Count	4
File Format	PDF
ISBN	9781424441211
ISSN	1557170X
e-ISBN	9781457715891
e-ISBN	9781424441228
DOI	10.1109/IEMBS.2011.6092071
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-08-30
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluids Microfluidics Fractionation Junctions Throughput Protocols Substrates
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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