NDLI: Real-time quantitative ex vivo direct autoradiography with 10μm pixel resolution

Content Provider	IEEE Xplore Digital Library
Author	Peng, Q. Holland, S.E. Choong, W.S. Budinger, T.F. Moses, W.W.
Copyright Year	2011
Description	Author affiliation: Lawrence Berkeley National Laboratory, Berkeley, CA 94720 USA (Peng, Q.; Holland, S.E.; Choong, W.S.; Budinger, T.F.; Moses, W.W.)
Abstract	We present three new autoradiography methods to map positron emission rate of a bio-specimen slice with high resolution. One is based on LBNL scientific charge coupled device (CCD) and the other two are based on conventional CCDs. High conversion efficiency (100k e-h pairs / 0.5MeV positron) and low dark current $(1.75×10^{−4}$ e-/pix/sec) can be achieved using the LBNL CCD. The theoretical calculations and preliminary experiments show that an 86μm spatial resolution can be achieved when imaging a 100 μm thick tissue soaked with $^{18}F$ which produce higher energy positron. The main disadvantage of the LBNL CCD we tested is that a very low operating temperature is required to eliminate dark current. This dramatically increases the system cost. In addition, the integration time of the CCD needs to be short enough to avoid overlapping of the positron trajectories. Conventional CCDs have lower conversion efficiency (2k e-h pairs / 0.5MeV positron) and higher dark current (200 e-/pix/sec), but are more cost-efficient and the requirement for the readout frequency is much lower. The conversion efficiency of the conventional CCD imager can be improved by 17 times by inserting a 100 μm layer of phosphor between the sample and the imager. However, the light emitted from the phosphor screen will be ∼100 μm diameter, which severely degrades the spatial resolution. A high readout frequency is also required to avoid the overlapping. The CCD systems designed in this study will be used to map positron emission rate of bio-specimens such as cancerous tissues acquired in regular biopsy procedure. They can also be used to corroborate tracer kinetic modeling at a cellular level.
Starting Page	6273
Ending Page	6276
File Size	1473631
Page Count	4
File Format	PDF
ISBN	9781424441211
ISSN	1557170X
e-ISBN	9781457715891
e-ISBN	9781424441228
DOI	10.1109/IEMBS.2011.6091548
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-08-30
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Charge coupled devices Positrons Spatial resolution Phosphors Dark current Trajectory Temperature Biopsy Charge Coupled Device Autoradiography Cancer
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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