NDLI: Non-invasive assessment for endothelial CD81 expression via targeted microbubbles

Content Provider	IEEE Xplore Digital Library
Author	Fei Yan Qiaofeng Jin Xin Liu Hairong Zheng
Copyright Year	2011
Description	Author affiliation: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of biomedical and Health Engineering, SIAT, Shenzhen 518055 China (Fei Yan; Qiaofeng Jin; Xin Liu; Hairong Zheng)
Abstract	The aim of our study was to investigate the use of targeted ultrasound microbubbles (MBs) for molecular imaging of murine endothelial CD81 expression. In the study, the anti-CD81-coated MBs was successfully prepared and characterizated. Murine bEnd.3 cells were stimulated with phenazine methosulfate (PMS) to induce the up-regulation of CD81 expression. Changes in CD81 expression after stimulation were tracked with anti-CD81-coated MBs and imaged by using SONIX RP ultrasound imaging system. Our results showed that endothelial CD81 expression was gradually up-regulated with the increase of PMS concentration. Correspondingly, the accumulation of targeted MBs was also gradually improved and could be inhibited competitively. The mean video intensity of stimulated cells from backscatter of the CD81-targeted MBs was significantly higher than that of the non-stimulated control (mean ± SD: 17.5 ± 3.6 versus 12.1 ± 2.9 pixel intensity; P < 0.01). In conclusion, CD81-targeted MBs allows non-invasive assessment of the expression levels of CD81 on the bEnd.3 cells and may provide potential insights into early atherosclerotic plaque detection and treatment monitoring using molecular ultrasound imaging.
Starting Page	7223
Ending Page	7225
File Size	423508
Page Count	3
File Format	PDF
ISBN	9781424441211
ISSN	1557170X
e-ISBN	9781457715891
e-ISBN	9781424441228
DOI	10.1109/IEMBS.2011.6091825
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-08-30
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Ultrasonic imaging Molecular imaging In vitro Atherosclerosis Proteins
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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