NDLI: Intracellular electroporation site distributions: Modeling examples for nsPEF and IRE pulse waveforms

Content Provider	IEEE Xplore Digital Library
Author	Gowrishankar, T. R. Esser, A. T. Smith, K. C. Son, R. S. Weaver, J. C.
Copyright Year	2011
Description	Author affiliation: Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA (Gowrishankar, T. R.; Esser, A. T.; Smith, K. C.; Son, R. S.; Weaver, J. C.)
Abstract	We illustrate expected electroporation (EP) responses to two classes of large electric field pulses by employing systems models, one of a cell in vitro and the other of multiple cells in vivo. The first pulse class involves “nsPEF” (nanosecond pulsed electric fields). The durations are less than a microsecond, but the magnitudes are extremely large, often 10 kV/cm or more, and all of the pores remain small. The second class involves “IRE” (irreversible electroporation). Durations are many microseconds to several milliseconds, but with magnitudes smaller than 10 kV/cm, and a wide range of pore sizes evolves. A key feature of both pulse classes is non-thermal cell killing by multiple pulses without delivering external drugs or genes. For small pulses the models respond passively (no pore creation) providing negative controls. For larger pulses transient aqueous pore populations evolve. These greatly increase local membrane conductance temporarily, causing rapid redistribution of fields near and within cells. This complex electrical behavior is generally not revealed by experiments reporting biological end points resulting from cumulative ionic and molecular transport through cell membranes. The underlying, heterogeneous pore population distributions are also not obtained from typical experiments. Further, traditional EP applications involving molecular delivery are usually assumed to create pores solely in the outer, plasma membrane (PM). In contrast, our examples support the occurrence of intracellular EP by both nsPEF and IRE, but with different intracellular spatial distributions of EP sites.
Starting Page	732
Ending Page	735
File Size	1689522
Page Count	4
File Format	PDF
ISBN	9781424441211
ISSN	1557170X
e-ISBN	9781457715891
e-ISBN	9781424441228
DOI	10.1109/IEMBS.2011.6090166
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-08-30
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Histograms Cancer In vivo Computational modeling Plasmas Erbium Tumors
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Intracellular electroporation site distributions: Modeling examples for nsPEF and IRE pulse waveforms

In silico estimates of cell electroporation by electrical incapacitation waveforms

Electrochemotherapy of solid tumors — Preclinical and clinical experience

Impedance Analyzer for in vivo Electroporation Studies

An architecture for integrating cancer model repositories

Electroporation based gene therapy — From the bench to the bedside

Mathematical models for absorption and efficacy of ovarian cancer treatments

Electrical conductivity changes during irreversible electroporation treatment of brain cancer

Dynamic mechanical finite element model of biological cells for studying cellular pattern formation

Intracellular electroporation site distributions: Modeling examples for nsPEF and IRE pulse waveforms

Similar Documents

Intracellular electroporation site distributions: Modeling examples for nsPEF and IRE pulse waveforms

In silico estimates of cell electroporation by electrical incapacitation waveforms

Electrochemotherapy of solid tumors — Preclinical and clinical experience

Impedance Analyzer for in vivo Electroporation Studies

An architecture for integrating cancer model repositories

Electroporation based gene therapy — From the bench to the bedside

Mathematical models for absorption and efficacy of ovarian cancer treatments

Electrical conductivity changes during irreversible electroporation treatment of brain cancer

Dynamic mechanical finite element model of biological cells for studying cellular pattern formation

Intracellular electroporation site distributions: Modeling examples for nsPEF and IRE pulse waveforms