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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ran, K. Zou, D. Q. Chang, Y. T. Ou, Y. W. Xiao, Y. Y. Li, Z. J. Duan, K. M. |
| Description | Country affiliation: China Author Affiliation: Ran K ( Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, China.); Zou DQ ( Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, China.); Xiao YY ( Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, China.); Chang YT ( Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, China.); Ou YW ( Department of Anesthesiology, The Third Xiangya Hospital, Central South University, Changsha, China.); Li ZJ ( Department of Anesthesiology, The Second Xiangya Hospital, Central South University, Changsha, China xiaoyanying_xyy@163.com.) |
| Abstract | This study aimed to investigate the protective effects and the mechanisms underlying these effects of isoflurane preconditioning in the delayed phase of myocardial ischemia-reperfusion injury. We randomly divided 30 healthy male New Zealand white rabbits into three groups with 10 rabbits in each group as follows: sham operation group (C group), ischemia-reperfusion group (I/R group), and 2.0% isoflurane preconditioning group (S group). Rabbits in the C group received thoracotomy for 160 min. Rabbits in the I/R group underwent left coronary artery occlusion for 40 min and reperfusion for 120 min. Rabbits in the S group received inhalation of 2.0% isoflurane and 100% oxygen for 2 h; after 24 h, rabbits in this group received the same treatment as that administered to rabbits in the I/R group. We examined the tumor necrosis factor alpha (TNF- ) levels in each group 20 min before occlusion of the left coronary, 20 and 40 min after occlusion of the left coronary artery, and 1 and 2 h after myocardial reperfusion. After reperfusion, immunoblotting was used to measure the myocardial caspase-3 expression levels, and the infarct size was measured using Evans blue and tetrazolium chloride staining. The levels of TNF- and caspase-3 were lower in the S group than in the I/R group, and the myocardial infarct size decreased in the S group. Thus, isoflurane preconditioning in the delayed phase exerted protective effects by decreasing the myocardial caspase-3 expression and TNF- production in a rabbit model of ischemia-reperfusion injury. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-07-03 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Caspase 3 Metabolism Ischemic Preconditioning Isoflurane Pharmacology Myocardial Reperfusion Injury Prevention & Control Tumor Necrosis Factor-alpha Animals Biosynthesis Models, Animal Myocardium Rabbits Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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