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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, X. K. Li, B. J. Sun, Y. J. |
| Description | Country affiliation: China Author Affiliation: Sun YJ ( Department of Pathology, Tianjin Fifth Central Hospital, Tangguc, Tianjin, China Yajingsun189@126.com.); Wang XK ( Department of Pathology, Tianjin Fifth Central Hospital, Tangguc, Tianjin, China.); Li BJ ( Department of Pathology, Tianjin Fifth Central Hospital, Tangguc, Tianjin, China.) |
| Abstract | We investigated the expression of S-phase kinase-associated protein 2 (SKP2) in breast cancer tissues, and the effects of SKP2-specific small interfering RNA (siRNA) interference on breast cancer cell proliferation. Thirty subjects provided breast cancer tissue samples and 18 subjects provided normal breast specimens for this study. The expression of SKP2 in breast cancer patient tissues and normal breast tissues was detected by western blotting analysis and reverse transcription-polymerase chain reaction. SKP2-specific siRNA was used to decrease SKP2 expression in breast cancer cell line MDA-MB-231. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to detect cell proliferation. SKP2 expression in breast cancer tissues was significantly higher than in normal breast tissues (P < 0.05). Two pairs of siRNA specific to SKP2 were required to downregulate SKP2 expression in the breast cancer cell line MDA-MB-231. The MTT assay showed that MDA-MB-231 growth significantly slowed after SKP2 interference. Patients with breast cancer have an increased SKP2 level. Interference in SKP2 gene expression can inhibit breast cancer cell growth, suggesting that SKP2 is potentially a new target for breast cancer therapy. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-08-10 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Breast Neoplasms Genetics Gene Expression S-phase Kinase-associated Proteins Metabolism Pathology Case-control Studies Cell Line, Tumor Cell Proliferation Neoplasm Staging Rna, Small Interfering Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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