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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, D. G. Liu, Q. Sun, D. Q. Sun, X. G. Li, J. L. Sun, X. B. |
| Description | Country affiliation: China Author Affiliation: Li DG ( Department of Pediatric Surgery, The Second Hospital of Shandong University, Jinan, China.); Li JL ( Department of Pediatric Surgery, The Second Hospital of Shandong University, Jinan, China.); Sun DQ ( Department of Pediatric Surgery, The Second Hospital of Shandong University, Jinan, China.); Sun XB ( Department of Pediatric Surgery, The Second Hospital of Shandong University, Jinan, China.); Sun XG ( Department of Pediatric Surgery, The Second Hospital of Shandong University, Jinan, China.); Liu Q ( Department of Pediatric Surgery, The Second Hospital of Shandong University, Jinan, China.) |
| Abstract | We investigated the mechanism of the effect of lentinan on 3T3-L1 fat cell formation by inhibiting the peroxisome proliferator-activated receptor gamma (PPARγ)/protein kinase B (AKT) signaling pathway. 3T3-L1 fat cells were treated with 80 mM lentinan with or without the PPARγ activator, 100 mM rosiglitazone for 24 h. Reverse transcription-polymerase chain reaction was applied to detect PPARγ and AKT mRNA expression levels. Western blotting was used to detect AKT protein expression level. Compared with the control group, 80 mM lentinan increased PPARγ mRNA expression and downregulated AKT mRNA expression. After treatment with rosiglitazone, PPARγ mRNA expression increased by 78% (P < 0.05), while AKT mRNA expression decreased by 71% (P < 0.05). Lentinan treatment decreased AKT protein expression by 33%, and AKT protein expression in the lentinan and rosiglitazone co-treatment group was reduced by 28% compared with the lentinan treatment group. We found that 80 mM lentinan increased PPARγ mRNA expression and reduced AKT mRNA. Combination treatment with rosiglitazone increased this effect. This suggests that lentinan can depress 3T3-L1 fat cell formation by inhibiting the PPARγ/AKT signaling pathway. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-07-17 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Adipocytes Cytology Lentinan Pharmacology Metabolism Proto-oncogene Proteins C-akt Signal Transduction Drug Effects 3t3-l1 Cells Animals Cell Differentiation Genetics Gene Expression Regulation Mice Rna, Messenger Thiazolidinediones Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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