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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ma, E. G. Zhang, Y. N. Liu, Y. B. Li, H. Li, X. L. Shen, W. X. |
| Description | Country affiliation: China Author Affiliation: Li XL ( The Cadre Ward, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.); Liu YB ( Department of Cardiology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.); Ma EG ( Department of Urinary Surgery, Harbin First Hospital, Harbin, China.); Shen WX ( Medical Oncology of Cardiology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.); Li H ( The Professional Judicial Police College of Heilongjiang, Health Clinic, Harbin, China.); Zhang YN ( The Cadre Ward, The Second Affiliated Hospital, Harbin Medical University, Harbin, China lixinglu3@126.com.) |
| Abstract | We investigated the synergistic effect of bone morphogenetic protein 9 (BMP9) and transforming growth factor (TGF)-b in the transformation of mesenchymal stem cells into osteoblasts. We evaluated the effect of BMP9 and TGF-b on the induction of osteoblast formation. Mitogen-activated protein kinase (MAPK) pathway-related proteins such as p38, extracellular receptor kinase 1/2, and c-Jun N-terminal kinase (JNK) were analyzed. The interactions between TGF-Smad and BMP-MAPK were also studied. BMP9 alone induced the differentiation of mesenchymal stem cells (MSCs) into osteoblasts and enhanced phosphorylation of p38, extracellular receptor kinase 1/2, and JNK. TGF-b alone failed to induce transformation, but could increase the effect of BMP9. In this process the activation of Smad resulted in activation of the JNK pathway in the MAPK pathway. BMP9 induced osteogenesis of MSC differentiation through the MAKP pathway, while TGF-b contributed to BMP9 enhancement through the Smad-JNK pathway. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-07-13 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Cell Differentiation Drug Effects Growth Differentiation Factor 2 Pharmacology Osteoblasts Cytology Transforming Growth Factor Beta Alkaline Phosphatase Metabolism Animals Cell Line Cell Proliferation Collagen Type I Extracellular Signal-regulated Map Kinases Jnk Mitogen-activated Protein Kinases Map Kinase Signaling System Mice Enzymology Osteopontin Phosphorylation Protein Kinase Inhibitors P38 Mitogen-activated Protein Kinases Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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