NDLI: Expression and antibody generation of the cancer-testis antigen, BIOT2-S.

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Year: 2016 Volume: 15

Year: 2016 Volume: 14

Year: 2015 Volume: 14

Year: 2015 Volume: 14 Issue: 4

Year: 2015 Volume: 14 Issue: 3

Effects of isoflurane preconditioning in the delayed phase on myocardial tumor necrosis factor alpha levels and caspase-3 protein expression in a rabbit model of ischemia-reperfusion injury.

Development of microsatellite markers for Hancornia speciosa Gomes (Apocynaceae).

MicroRNAs as novel biomarkers for the differentiation of malignant versus benign thyroid lesions: a meta-analysis.

Correlation of CD4â º CD25â º Foxp3â º Treg with the recovery of joint function after total knee replacement in rats with osteoarthritis.

Biotechnological prospecting of foliar endophytic fungi of guaco (Mikania glomerata Spreng.) with antibacterial and antagonistic activity against phytopathogens.

Allele frequencies of combined DNA index system (CODIS) and non-CODIS short tandem repeat loci in Goiás, Central Brazil.

Wnt1-induced MAFK expression promotes osteosarcoma cell proliferation.

Genetic polymorphism in sex hormone metabolism and prostate cancer risk.

Phenotypic correlations between ovum pick-up in vitro production traits and pregnancy rates in Zebu cows.

Functional molecular markers (EST-SSR) in the full-sib reciprocal recurrent selection program of maize (Zea mays L.).

Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing.

Codon usage bias analysis for the spermidine synthase gene from Camellia sinensis (L.) O. Kuntze.

Correlation between the BRAF V600E mutation status and the clinicopathologic features of papillary thyroid carcinoma.

Temporal and tissue expression of genes involved in buds of earliness cotton cultivar.

rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco.

Characterization and expression of the calpastatin gene in Cyprinus carpio.

Genetic diversity in intraspecific hybrid populations of Eucommia ulmoides Oliver evaluated from ISSR and SRAP molecular marker analysis.

Meta-analysis of the association between the HNF1B rs4430796 (A>G) polymorphism and risk of prostate cancer based on case-control studies.

Anastomosis groups and molecular variation in Pseudocercospora griseola.

Forkhead box protein O1 mediates apoptosis in a cancer cervical cell line treated with the antitumor agent tumor necrosis factor- .

Meiotic behavior and chromosome number of Urochloa adspersa (Trin.) R. D. Webster from the Brazilian Chaco.

Boswellic acid attenuates asthma phenotype by downregulation of GATA3 via nhibition of PSTAT6.

Transcriptomic identification of chemoreceptor genes in the red palm weevil Rhynchophorus ferrugineus.

Effect of melatonin on oncosis of myocardial cells in the myocardial ischemia/reperfusion injury rat and the role of the mitochondrial permeability transition pore.

Proton pump inhibitor use and risk of spontaneous bacterial peritonitis in cirrhotic patients: a systematic review and meta-analysis.

Genetic diversity, population structure, and association mapping of agronomic traits in waxy and normal maize inbred lines.

Treatment of Moyamoya disease by multipoint skull drilling for indirect revascularization combined with mobilization of autologous bone marrow stem cells.

Propofol suppresses proliferation and invasion of pancreatic cancer cells by upregulating microRNA-133a expression.

Effects of 6-week specific low-intensity training on selected aerobic capacity parameters and HSPA1A, HSPB1, and LDHb gene expression in high-level rowers.

Inhibitory effects of a dendritic cell vaccine loaded with radiation-induced apoptotic tumor cells on tumor cell antigens in mouse bladder cancer.

Genetic diversity analysis of tree peony germplasm using iPBS markers.

Endophytic bacteria from Piper tuberculatum Jacq.: isolation, molecular characterization, and in vitro screening for the control of Fusarium solani f. sp piperis, the causal agent of root rot disease in black pepper (Piper nigrum L.).

Development of expressed sequence tag-simple sequence repeat markers for Chrysanthemum morifolium and closely related species.

Genetic diversity in Tunisian populations of faba bean (Vicia faba L.) based on morphological traits and molecular markers.

Bispectral index for monitoring anesthetic depth in patients with severe burns receiving target-controlled infusion of remifentanil and propofol.

Synergistic effect of BMP9 and TGF-ß in the proliferation and differentiation of osteoblasts.

Radiofrequency thermocoagulation combined with pulsed radiofrequency helps relieve postoperative complications of trigeminal neuralgia.

Isolation and characterization of microsatellite loci in the purpleback flying squid (Sthenoteuthis oualaniensi).

Predictive value of vascular endothelial growth factor polymorphisms on the risk of renal cell carcinomas.

Branches of NF-κb signaling pathway regulate hepatocyte proliferation in rat liver regeneration.

Association between polymorphisms in insulin-like growth factor-1 and risk of osteoporosis.

Purification of the insecticidal Cry2Ad protein from a Bt-isolated BRC-HZP10 strain and toxin assay to the diamondback moth, Plutella xylostella (L.).

Fructus polygoni orentalis extract inhibited liver regeneration and proliferation of bone marrow cells of rat after partial hepatectomy.

Association between the hsa-miR-146a rs2910164 functional polymorphism with susceptibility to intracranial aneurysm.

Accumulation of flavanols and expression of leucoanthocyanidin reductase induced by postharvest UV-C irradiation in grape berry.

Neuroprotective effects of Bcl-2 overexpression on nerve cells of rats with acute cerebral infarction.

Effects of sex and age on chicken TBC1D1 gene mRNA expression.

Development of novel chloroplast microsatellite markers for Ginkgo biloba.

Stimulation of bacterial biofilms on Th17 immune cells.

Genetic diversity of the endemic flat-needle pine Pinus krempfii (Pinaceae) from Vietnam revealed by SSR markers.

Molecular cloning, characterization, and expression of Rab5B, Rab6A, and Rab7 from Litopenaeus vannamei (Penaeidae).

Relationship between a lipoprotein lipase gene polymorphism in placental tissue and insulin resistance in patients with gestational diabetes mellitus.

Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers.

An improved K-means clustering method for cDNA microarray image segmentation.

A new method to induce myasthenia gravis models and the protective effect of soluble decay accelerating factors.

Large-scale detection and application of expressed sequence tag single nucleotide polymorphisms in Nicotiana.

Effect of GBOT on blood lipid and blood glucose metabolism in rats with atherosclerosis.

Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum.

An expression profiling analysis of hybrid millet and its parents at grain filling stage.

Replication timing regulation in adults with chromosomal balance rearrangements.

Differentiation-inducing effects of betamethasone on human glioma cell line U251.

Heterogeneity and diversity of ABO and Rh blood group genes in select Saudi Arabian populations.

Manipulation of primer affinity improves high-resolution melting accuracy for imprinted genes.

Effect of skeletal muscle fibers on porcine meat quality at different stages of growth.

Expression differences in TEL-AML1 fusion gene in leukemia glucocorticoid-sensitive and -resistant cell lines.

Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers.

Rapid development of polymorphic microsatellite markers for the Amur sturgeon (Acipenser schrenckii) using next-generation sequencing technology.

Development of EST-SSR and TRAP markers from transcriptome sequencing data of the mango.

Association between the rs3795879 G/A polymorphism of the SERPINE2 gene and chronic obstructive pulmonary disease: a meta-analysis.

Pathology of the lower nasolacrimal duct mucosa at multiple time points after lacrimal retrograde catheterization in rabbit.

Transferability of microsatellite markers of Capsicum annuum L. to C. frutescens L. and C. chinense Jacq.

In silico analysis of gene content in tomato genomic regions mapped to the Ty-2 resistance gene.

Reduced cortisol in the absence of bacterial infection in patients with hepatitis B virus cirrhosis.

Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape.

Relationship between UV-irradiated HaCaT cell cytokines and Th1/Th2 imbalance.

Characterization of microsatellite markers for the Restinga Antwren, Formicivora littoralis (Thamnophilidae), an endangered bird endemic to Brazil.

Characterization of FeDREB1 promoter involved in cold- and drought-inducible expression from common buckwheat (Fagopyrum esculentum).

A simple, fast, and inexpensive CTAB-PVP-silica based method for genomic DNA isolation from single, small insect larvae and pupae.

Molecular cloning, characterization, and expression of vacuolar-type-Hâ º-ATPase B1 (VHAB1) gene in the gill of Anguilla marmorata.

Correlation between MTHFR gene methylation and pre-eclampsia, and its clinical significance.

Protective effect and mechanism of probucol in the treatment of spinal cord injury.

Screening of differentially-expressed genes in the muscles of rabbit breeds with expression profile chip.

Estimation of genetic structure of a Mycosphaerella musicola population using inter-simple sequence repeat markers.

Genetic structure and genetic diversity of single-variety Lonicera macranthoides populations in China, as indicated by SCoT markers.

Meta-analysis of angiotensin-converting enzyme insertion/deletion polymorphism and myocardial infarction in Han Chinese.

Stripe rust resistance and dough quality of new wheat - Dasypyrum villosum translocation lines T1DLâ ¢1V#3S and T1DSâ ¢1V#3L and the location of HMW-GS genes.

Lentinan depresses 3T3-L1 fat cell formation by inhibiting PPARÎ³/AKT signaling pathway.

Correlation of plasma soluble cluster of differentiation 40 ligand, alpha fetoprotein A, and pregnancy-associated plasma protein A with carotid plaque in patients with ischemic stroke.

Selection and molecular identification of fungal isolates that produce xylanolytic enzymes.

Propofol suppresses proliferation and invasion of gastric cancer cells via downregulation of microRNA-221 expression.

Impacts of transgenic poplar-cotton agro-ecosystems upon target pests and non-target insects under field conditions.

Anti-osteoporosis activity of red yeast rice extract on ovariectomy-induced bone loss in rats.

TGF-ß1 polymorphisms and familial aggregation of liver cancer in Guangxi, China.

Mendelian inheritance, genetic linkage, and genotypic disequilibrium at microsatellite loci in Genipa americana L. (Rubiaceae).

Influence of plasma exchange on transplantation related immune function in patients with liver failure.

Expression of drought tolerance genes in tropical upland rice cultivars (Oryza sativa).

Identification of Pseudomonas syringae pv. actinidiae strains causing bacterial canker of kiwifruit in the Anhui Province of China, and determination of their streptomycin sensitivities.

MTHFR A1298C polymorphism and ovarian cancer risk: a meta-analysis.

Effect of generations and environments in the analysis of a partial diallel to improve bean earliness.

Application of indirect linkage analysis and direct genotyping to hemophilia A carrier detection in Sichuan, China.

Evaluation of progenies from the fifth reciprocal recurrent selection cycle in maize.

Treatment and mechanism of BMMSCs on deep II degree scald of hamster skin.

Relationship between polymorphisms in exon 10 of FSHR gene and litter size in swine.

Expression and localization of cFLIP anti-apoptotic protein in the porcine corpus luteum and corpora albicans during the estrous cycle and pregnancy.

Association of the genetic polymorphisms of NFKB1 with susceptibility to ovarian cancer.

Investigation of the role of VEGF gene polymorphisms in the risk of osteosarcoma.

Development and characterization of microsatellite markers for molecular genetic diversity in Atrina pectinata.

Expression of LTP genes in response to saline stress in rice seedlings.

Human chorionic gonadotropin promotes expression of protein absorption factors in the intestine of goldfish (Carassius auratus).

Leaf transcriptome analysis and development of SSR markers in water chestnut (Eleocharis dulcis).

Characterization of NF1 frameshift mutations in pediatric patients with neurofibromatosis type I.

Potassium contributes to zinc stress tolerance in peach (Prunus persica) seedlings by enhancing photosynthesis and the antioxidant defense system.

Relationship between urinary protein changes in lupus nephritis and renal pathology.

Transcriptome sequencing and analysis of the coconut leaf beetle, Brontispa longissima.

Physical localization of molecular markers and assignment of the 15th linkage group to chromosome 11 of the karyotype in cassava (Manihot esculenta Crantz) by primed in situ labeling.

Association between five common polymorphisms in microRNA genes and the risk of gastric cancer: a meta-analysis.

Verification of specific selection SNPs between broiler and layer chicken in Chinese indigenous chicken breeds.

Construction of a natural phage antibody library of human anaplastic thyroid carcinoma.

Genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China revealed by simple-sequence repeat markers.

Sequence variation in the Toxoplasma gondii ROP20 gene among strains from different hosts and geographical locations.

Non-parental banding patterns in recombinant inbred line population of maize with SSR markers.

Identification of heat shock proteins via transcriptome profiling of tree peony leaf exposed to high temperature.

Promoter and first exon methylation regulate porcine FASN gene expression.

IL-6-174G/C and IL-6-572C/G polymorphisms are associated with increased risk of coronary artery disease.

Regulators of G-protein signaling 9 genetic variations in Chinese subjects with schizophrenia.

Immune memory responses to HBV vaccine 13-18 years after primary vaccination.

CYP19 gene polymorphisms and the susceptibility to breast cancer in Xinjiang Uigur women.

Production of transgenic kiwifruit plants harboring the SbtCry1Ac gene.

Lack of association between an insertion/deletion polymorphism in IL1A and risk of colorectal cancer.

Isolation and characterization of microsatellite markers of sea cucumber Stichopus horrens.

Polymorphisms in the methylene tetrahydrofolate reductase and methionine synthase reductase genes and their correlation with unexplained recurrent spontaneous abortion susceptibility.

Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA.

Interleukin-6 gene -174G>C polymorphism and chronic obstructive pulmonary disease risk: a meta-analysis.

Influence of interleukin-17 gene polymorphisms on the development of pulmonary tuberculosis.

-173G/C polymorphism in the promoter of MIF is associated with hepatitis B virus infection in a Chinese Han population.

Related factors and prevalence of Parkinson's disease among Uygur residents in Hetian, Xinjiang Uygur Autonomous Region.

Smoking, aging, and expression of proteins related to the FOXO3 signaling pathway in lung tissues.

Curative effect and costs of surgical and gamma knife treatments on intractable epilepsy caused by temporal-hippocampal sclerosis.

Adjuvant chemotherapy may improve survival of patients with luminal A breast cancer and positive lymph nodes.

Cloning and expression of the porcine attaching and effacing-associated (paa) gene of enteropathogenic Escherichia coli.

Novel SNPs in the growth arrest and DNA damage-inducible protein 45 alpha gene (GADD45A) associated with meat quality traits in Berkshire pigs.

Common polymorphisms of the microRNA genes (miR-146a and miR-196a-2) and gastric cancer risk: an updated meta-analysis.

Associations between tumor necrosis factor- polymorphisms and susceptibility to pulmonary tuberculosis: meta-analysis.

Mn-SOD 47 CC genotype in combination with high tea consumption may prevent complications in Tunisian type-2 diabetes.

Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds.

Study on the correlation between the expression of Ki67 and FasL and prognosis of cervical carcinoma.

Cytogenetic characterization of species of the family Heptapteridae (Teleostei: Siluriformes) from the Cuiabá and Ivaí River Basins, Brazil.

mRNA expression of prolactin receptor in sheep pituitary and its effect on maternal behavior.

Detection of Toxoplasma gondii DNA in naturally infected sheep's milk.

Consistency between molecular phylogeny and morphological classification of the Salix matsudana Koidz. complex (Salicaceae).

Comparative analysis of laparoscopic and ultrasound-guided biopsy methods for gene expression analysis in transgenic goats.

Expression and antibody generation of the cancer-testis antigen, BIOT2-S.

Association of TLR2 and TLR4 non-missense single nucleotide polymorphisms with type 2 diabetes risk in a southern Chinese population: a case-control study.

Dynamic QTL analysis of protein content and glutamine synthetase activity in recombinant inbred wheat lines.

Correlation between the decrease of cholesterol efflux from macrophages in patients with type II diabetes mellitus and down-regulated CYP7A1 expression.

Transplantation of umbilical cord blood mononuclear cells increases levels of nerve growth factor in the cerebrospinal fluid of patients with autism.

A fast neighbor joining method.

Effect of bone marrow mesenchymal stem cells on the TGF-ß1/Smad signaling pathway of hepatic stellate.

A cytological study of anther and pollen development in Camellia oleifera.

Downregulation of microRNA-630 inhibits cell proliferation and invasion and enhances chemosensitivity in human ovarian carcinoma.

Association between the rs4753426 polymorphism in MTNR1B with fasting plasma glucose level and pancreatic ß-cell function in gestational diabetes mellitus.

Correlation between natriuretic peptide receptor C (NPR3) gene polymorphisms and hypertension in the Dai people of China.

Effect of HIF-1a/VEGF signaling pathway on plasma progesterone and ovarian prostaglandin F2a secretion during luteal development of pseudopregnant rats.

Characterization and effects of miR-21 expression in esophageal cancer.

Insulin receptor binding motif tagged with IgG4 Fc (Yiminsu) works as an insulin sensitizer to activate Akt signaling in hepatocytes.

Biogeographic pattern of genetic diversity detected by RAPD and ISSR analysis in Gypsophila (Caryophyllaceae) species from Turkey.

Protein expression levels in the medullary visceral zone of rats with subarachnoid hemorrhage.

Characterization and expression analysis of the prophenoloxidase activating factor from the mud crab Scylla paramamosain.

Impact of adherence to GOLD guidelines on 6-minute walk distance, MRC dyspnea scale score, lung function decline, quality of life, and quality-adjusted life years in a Shanghai suburb.

Prediction of core cancer genes using a hybrid of feature selection and machine learning methods.

Role of adiponectin in adipose tissue wound healing.

Pdxl and its role in activating Ngn3 and Pax6 to induce differentiation of iPSCs into islet ß cells.

Effect of montelukast on the expression of interleukin-18, telomerase reverse transcriptase, and Bcl-2 in the brain tissue of neonatal rats with hypoxic-ischemic brain damage.

Vascular endothelial growth factor +405G/C and -2578C/A polymorphisms and breast cancer risk: a meta-analysis.

Serum YKL-40 levels are associated with type 2 diabetes mellitus in patients with obstructive sleep apnea syndrome.

Characterization of microsatellite markers and their correlations with growth traits in Mandarin fish (Siniperca chuatsi).

Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat.

Protective effects of morphine preconditioning in delayed phase on myocardial ischemia-reperfusion injury in rabbits.

Synonymous codon selection in the hepatitis B virus translation initiation region.

Complete mitochondrial genome of the Chinese Hwamei Garrulax canorus (Aves: Passeriformes): the first representative of the Leiothrichidae family with a duplicated control region.

Development of novel polymorphic microsatellite markers for the blood clam Tegillarca granosa by pyrosequencing.

Study of nucleolar behavior during spermatogenesis in Martarega brasiliensis (Heteroptera, Notonectidae).

Adaptability of soybean cultivars in different crop years.

Associations between the SRD5A2 gene V89L and TA repeat polymorphisms and breast cancer risk: a meta-analysis.

Polymorphisms in the bovine CIDEC gene are associated with body measurement traits and meat quality traits in Qinchuan cattle.

Association of the p53 Arg72Pro polymorphism with esophageal cancer in Chinese populations: a meta-analysis.

Association of MyoD1a and MyoD1b gene polymorphisms and meat quality traits in rainbow trout.

Sex identification based on AMEL gene PCR amplification from blue sheep (Pseudois nayaur) fecal DNA samples.

Associations of single nucleotide polymorphisms in the Pygo2 coding sequence with idiopathic oligospermia and azoospermia.

Role of inflammatory responses in the pathogenesis of human cerebral aneurysm.

Use of microsatellite markers to assign goats to their breeds.

MicroRNAs in avian influenza virus H9N2-infected and non-infected chicken embryo fibroblasts.

Association between 1p13.3 genomic markers and coronary artery disease: a meta-analysis involving patients and controls.

Computational identification and characterization of conserved miRNAs and their target genes in beet (Beta vulgaris).

Local expression and role of BMP-2/4 in injured spinal cord.

Vitamin D receptor gene FokI, TaqI, BsmI, and ApaI polymorphisms and susceptibility to pulmonary tuberculosis: a meta-analysis.

Effects of different anesthetic approaches on postoperative complications in HIV-infected patients.

Association between rs155971 in the PCSK1 gene and the lipid profile of obese Thai children: a family-based study.

Regulation of CD4â ºFOXP3â º T cells by CCL20/CCR6 axis in early unexplained recurrent miscarriage patients.

Macrophage proinflammatory response to the titanium alloy equipment in dental implantation.

Association of AGTR1 gene A1166C polymorphism with the risk of heart failure: a meta-analysis.

Effects of abnormal optineurin expression on the survival of the rat retinal ganglion cell line RGC-5.

Application of DNA barcoding markers to the identification of Hopea species.

Characteristic element of matrix attachment region mediates vector attachment and enhances nerve growth factor expression in Chinese hamster ovary cells.

Genome-scale meta-analysis of DNA methylation during progression of lung adenocarcinoma.

Opg/Rankl mRNA dynamic expression in the bone tissue of ovariectomized rats with osteoporosis.

Expression of human amyloid precursor protein in Drosophila melanogaster nerve cells causes a decrease in presynaptic gene mRNA levels.

Aspergillus fumigatus diffusates suppress polymorphonuclear neutrophil phagocytic functions and respiratory burst levels in hematopoietic stem cell transplantation patients.

S-phase kinase-associated protein 2 expression interference inhibits breast cancer cell proliferation.

Genetic diversity and taxonomic status of Gymnocypris chilianensis based on the mitochondrial DNA cytochrome b gene.

Investigation of polymorphisms in exon7 of the NSUN7 gene among Chinese Han men with asthenospermia.

Telomere-associated factor expression in replicative senescence of human embryonic lung fibroblasts.

Relationship of the APOA5/A4/C3/A1 gene cluster and APOB gene polymorphisms with dyslipidemia.

Cloning and transformation analysis of isoflavone synthase gene into Minshan Trifolium pratense.

Highly specific detection of genetic modification events using an enzyme-linked probe hybridization chip.

Ectopic expression of the BoTFL1-like gene of Bambusa oldhamii delays blossoming in Arabidopsis thaliana and rescues the tfl1 mutant phenotype.

New compound heterozygous mutations of p. Thr101Ilefs 2 and p. Thr306Ale in a child from a Chinese family with 17 -hydroxylase/17, 20-lyase deficiency.

Effect of phosphatidylserine on memory in patients and rats with Alzheimer's disease.

Protective effect of penehyclidine hydrochloride on lipopolysaccharide-induced acute kidney injury in rat.

Simple sequence repeat-based analysis of the genetic diversity and population genetic structure of populations of Siniperca chuatsi.

Effect of baculovirus P35 protein on apoptosis in brain tissue of rats with acute cerebral infarction.

Estrogen receptor 1 PvuII and XbaI polymorphisms and susceptibility to Alzheimer's disease: a meta-analysis.

C/EBP gene as a genetic marker for beef quality improvement.

P1BS, a conserved motif involved in tolerance to phosphate starvation in soybean.

Frequency of SNP -336A/G in the promoter region of CD209 in a population from northeastern Brazil.

Association of the VRK2 gene rs3732136 polymorphism with schizophrenia in a Northwest Chinese Han population.

In silico identification and analysis of phytoene synthase genes in plants.

Analysis of alterations to the transcriptome of Loquat (Eriobotrya japonica Lindl.) under low temperature stress via de novo sequencing.

Isolation and characterization of novel polymorphic microsatellite loci in large yellow croaker (Larimichthys crocea).

Treating congenital megacolon by transplanting GDNF and GFR -1 double genetically modified rat bone marrow mesenchymal stem cells.

Molecular and chemical characterization of vetiver, Chrysopogon zizanioides (L.) Roberty, germplasm.

Efficient construction of recombinant adenovirus expression vector of the Qinchuan cattle LYRM1 gene.

N-ethylmaleimide-sensitive factor siRNA improves cardiac function following myocardial infarction in rats.

Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells.

Molecular analysis and conventional cytology: association between HPV and bacterial vaginosis in the cervical abnormalities of a Brazilian population.

OsMYB511 encodes a MYB domain transcription activator early regulated by abiotic stress in rice.

Amiloride, a urokinase-type plasminogen activator receptor (uTPA) inhibitor, reduces proteinurea in podocytes.

Specialized features of the outer hair cell shapes in the cochlear fovea of bats.

Estimating genetic potential of biofuel forest hardwoods to withstand metal toxicity in industrial effluent under dry tropical conditions.

Assessment of genotoxicity of waterpipe smoking using 8-OHdG biomarker.

Effect of hypothalamic-pituitary-adrenal axis alterations on glucose and lipid metabolism in diabetic rats.

Pathway-based network analysis of myeloma tumors: monoclonal gammopathy of unknown significance, smoldering multiple myeloma, and multiple myeloma.

Lack of association between potential prothrombotic genetic risk factors and arterial and venous thrombosis.

Association between IL-21 polymorphism and systemic lupus erythematosus: a meta-analysis.

Mechanism of rat osteosarcoma cell apoptosis induced by a combination of low-intensity ultrasound and 5-aminolevulinic acid in vitro.

MicroRNA profiling in cutaneous wounds of diabetic rats.

Association between single nucleotide polymorphisms of X-ray repair cross-complementing protein 4 gene and development of pancreatic cancer.

Adaptability and stability of soybean genotypes in off-season cultivation.

Estimating the genetic parameters for liver fat traits in broiler lines divergently selected for abdominal fat.

Human kallikrein 5 as a novel prognostic biomarker for triple-negative breast cancer: tissue expression analysis and relationship with disease course.

Polymorphisms in the promoter regions of the CXCL1 and CXCL2 genes contribute to increased risk of alopecia areata in the Korean population.

Silencing FKBP38 gene by siRNA induces activation of mTOR signaling in goat fetal fibroblasts.

Potential of berberine to enhance antimicrobial activity of commonly used antibiotics for dairy cow mastitis caused by multiple drug-resistant Staphylococcus epidermidis infection.

Analysis of the genetic diversity of beach plums by simple sequence repeat markers.

In silico analysis of the 16S rRNA gene of endophytic bacteria, isolated from the aerial parts and seeds of important agricultural crops.

Genetic diversity analysis of sweet kernel apricot in China based on SSR and ISSR markers.

Relationship between zinc and the growth and development of young children.

Effects of acupotomylysis on basic fibroblast growth factor and CD34 levels in rabbits with third lumbar vertebral transverse foramen syndrome.

Antimutagenic action of the triterpene betulinic acid isolated from Scoparia dulcis (Scrophulariaceae).

Identification of target genes for adenohypophysis-prefer miR-7 and miR-375 in cattle.

Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library.

Identification of conservation units of the hynobiid salamander Pachyhynobius shangchengensis.

Expression of genes coding for selected amino acid transporters in small intestine, liver, and skeletal muscle of pigs fed excess branched-chain amino acids.

Population structure and genetic diversity in Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae): is there a correlation between genetic and geographic proximity?

CpG ODN 1826 enhances radiosensitivity of the human lung cancer cell line A549 in a rat model.

Study of the methylation patterns of the EGFR gene promoter in non-small cell lung cancer.

Association between the XRCC1 Arg399Gln polymorphism and risk of cervical carcinoma: a meta-analysis.

Genetic diversity of popcorn genotypes using molecular analysis.

Analysis of the expression of HMGB-1, CXCL16, miRNA-30a, and TGF-ß1 in primary nephritic syndrome patients and its significance.

Vascular endothelial growth factor gene is associated with hypertensive cerebellar hemorrhage and rehabilitative treatment.

Genetic analysis of maize kernel thickness by quantitative trait locus identification.

Comparison of high-intensity focused ultrasound therapy under nasal endoscopy guidance versus first-line drug treatment in patients with persistent allergic rhinitis.

Quantitative trait locus analysis of grape weight and soluble solid content.

Expression of N-cadherin in myocardial tissues during the development of a rat heart.

Phylogeography of pink pineapple mealybugs, Dysmicoccus brevipes (Cockerell) reveals the history of pineapple introduction and cultivation in China.

Superiority of artificial neural networks for a genetic classification procedure.

Screening of basidiomycetes in submerged cultivation based on antioxidant activity.

SLC2A9 and ZNF518B polymorphisms correlate with gout-related metabolic indices in Chinese Tibetan populations.

Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana.

Operational taxonomic units (OTUs) of endophytic bacteria isolated from banana cultivars in the Amazon.

Polymorphic microsatellite markers in the traditional Chinese medicinal plant Paris polyphylla var. yunnanensis.

Proline accumulation is a general response to abiotic stress in the date palm tree (Phoenix dactylifera L.).

In vivo determination of muscle-derived stem cells in rat corpus cavernosum.

Evolutionary analysis of the ubiquitin gene of baculovirus and insect hosts.

Genetic diversity among different physiological traits of Sorghum bicolor cultivars of subtropical origin.

Differential expression of luteinizing hormone receptor, androgen receptor and heat-shock protein 70 in the testis of long-distance transported mice.

PI3K-AKT pathway polymerase chain reaction (PCR) array analysis of epilepsy induced by type II focal cortical dysplasia.

Thymidylate synthase and methylenetetrahy-drofolate reductase gene polymorphisms and gastric cancer susceptibility in a population of Northern Brazil.

Effect of continuous infusion of midazolam on immune function in pediatric patients after surgery.

Effects of microwave ablation or its combination with whole-body chemotherapy on serum vascular endothelial growth factor levels in patients with stage IIIB/IV NSCLC.

Association of variants in renal salt reabsorption-related gene SLC12A3 with essential hypertension in a Mongolian population.

Cloning and characterization of DELLA genes in Artemisia annua.

Effect of Bifidobacterium on the mRNA expression levels of TRAF6, GSK-3ß, and microRNA-146a in LPS-stimulated rat intestinal epithelial cells.

Analysis of the relationship between peripheral blood T lymphocyte subsets and HCV RNA levels in patients with chronic hepatitis C.

Association of TCF7L2 gene polymorphisms with susceptibility to type 2 diabetes mellitus in a Chinese Hui population.

Expression patterns of melatonin receptors in chicken ovarian follicles affected by monochromatic light.

Association between PAI-1 polymorphisms and plasma PAI-1 level with sepsis in severely burned patients.

Antiviral effect of hepatitis B virus S/C gene loci antisense locked nucleic acid on transgenic mice in vivo.

Role of autoantibodies to various Ro60 epitopes in the decrease of lymphocytes seen in systemic lupus erythematosus and primary Sjögren's syndrome.

Comparative proteomic analysis reveals mite (Varroa destructor) resistance-related proteins in Eastern honeybees (Apis cerana).

Identification and analysis of the TIFY gene family in Gossypium raimondii.

Superoxide radical-generating compounds activate a predicted promoter site for paraquat-inducible genes of the Chromobacterium violaceum bacterium in a dose-dependent manner.

Association between ERCC1 and ERCC2 gene polymorphisms and chemotherapy response and overall survival in osteosarcoma.

Differential expression of TLP, ERF1, and R2R3MYB in annual Medicago species under salinity conditions.

Relevance of sampling and DNA extraction techniques for the analysis of salivary evidence from bite marks: a case report.

Effect of DNase treatment on RNA extraction from preimplantation murine embryos.

Differential expression of glypican-3 (GPC3) in lung squamous cell carcinoma and lung adenocarcinoma and its clinical significance.

Investigation of genes in chronic and acute morphine-treated mice using microarray datasets.

Cloning and stress response analysis of the PeDREB2A and PeDREB1A genes in moso bamboo (Phyllostachys edulis).

Isolation and characterization of microsatellite loci in Branchiostoma belcheri Gray (Amphioxus).

A clinical study evaluating dendritic and cytokine-induced killer cells combined with concurrent radiochemotherapy for stage IIIB non-small cell lung cancer.

Predictive potential role of glutathione S-transferase polymorphisms in the prognosis of breast cancer.

Duplication polymorphisms in exon 4 of κ-casein gene in yak breeds/populations.

Genomic characterization and sequence diversity of the ß2-microglobulin gene in the miiuy croaker, Miichthys miiuy.

Androgen receptor (CAG)n polymorphisms and breast cancer risk in a Han Chinese population.

Inheritance of balanced translocation t(17; 22) from a Down syndrome mother to a phenotypically normal daughter.

Influence of the DCC gene on proliferation and carcinoembryonic antigen expression in the human colorectal cancer cell line SW1116.

Investigation of single nucleotide polymorphisms in phosphodiesterase 4D gene in Mongol and Han patients with ischemic stroke in Inner Mongolia.

Integrated microRNA-mRNA analysis of pancreatic ductal adenocarcinoma.

Populations of Erythrina velutina Willd. at risk of extinction.

Screening and identification of microsatellite markers associated with cold tolerance in Nile tilapia Oreochromis niloticus.

Inhibitory effects of spironolactone on myocardial fibrosis in spontaneously hypertensive rats.

HLA-A gene polymorphisms contribute to osteoporosis susceptibility in postmenopausal Han Chinese women.

Association of GSTP1 and XRCC1 gene polymorphisms with clinical outcomes of patients with advanced non-small cell lung cancer.

Expression and clinical significance of CD4+ CD45+ peripheral blood T cells in patients with ulcerative colitis.

EDA mutation as a cause of hypohidrotic ectodermal dysplasia: a case report and review of the literature.

Detecting circulating tumor cells in patients with advanced non-small cell lung cancer.

Effects of silicon on seed setting rate of rice intersubspecific hybrids.

Genetic structure of natural populations of Theobroma in the Juruena National Park, Mato Grosso State, Brazil.

Roles of the AIB1 protein in the proliferation and transformation of human esophageal squamous cell carcinoma.

Correlations among copeptin, ischemia-modified albumin, and the extent of myocardial injury in patients with acute carbon monoxide poisoning.

Interactome analysis and design of inhibitors against selected protein targets of Ser/Thr protein kinase (STPK) signaling pathways in Mycobacterium tuberculosis H37Rv.

Mapping quantitative trait loci for nitrogen uptake and utilization efficiency in rice (Oryza sativa L.) at different nitrogen fertilizer levels.

Association between polymorphisms of the CRH and POMC genes with economic traits in Korean cattle (Hanwoo).

Conservation and population genetic diversity of Curcuma wenyujin (Zingiberaceae), a multifunctional medicinal herb.

Overexpression of the CmACS-3 gene in melon causes abnormal pollen development.

Heterosis for the improvement of yield in mungbean [Vigna radiata (L.) Wilczek].

Genome-wide association study of meat quality traits in chicken.

Polymorphism and haplotype analyses of swine leukocyte antigen DQA exons 2, 3, 4, and their associations with piglet diarrhea in Chinese native pig.

Correlation of E6 and E7 levels in high-risk HPV16 type cervical lesions with CCL20 and Langerhans cells.

Effect of chronic hypoxia on penile erectile function in rats.

Effect of low temperatures on BAX and BCL2 proteins in rats with spinal cord ischemia reperfusion injury.

Tissue- and breed-specific expression of the chicken fat mass- and obesity-associated gene (FTO).

Relationship between vitamin D receptor (VDR) polymorphisms and the efficacy of recombinant human growth hormone (rhGH) treatment in children with idiopathic short stature.

DAP1 high expression increases risk of lymph node metastases in squamous cell carcinoma of the oral cavity.

Association between angiogenic growth factor genetic polymorphisms and the risk of osteosarcoma.

Identification and evaluation of endogenous control genes for use in quantitative RT-PCR during wheat (Triticum aestivum L.) grain filling.

Elucidating the function and tolerance mechanism of gamma delta (Î³ Î´) T cells in a Helicobacter pylori infection model.

Inhibitory effect of berberine on human skin squamous cell carcinoma A431 cells.

DNA repair gene XRCC3 variants are associated with susceptibility to glioma in a Chinese population.

Evidence of genetic tolerance to low availability of phosphorus in the soil among genotypes of Coffea canephora.

Genetic bottlenecks in Turkish okra germplasm and utility of iPBS retrotransposon markers for genetic diversity assessment.

Systematic review of noninvasive prenatal diagnosis for abnormal chromosome genetic diseases using free fetal DNA in maternal plasma.

Inheritance and quantitative trait locus analysis of low-light tolerance in cucumber (Cucumis sativus L.).

GlpC gene is responsible for biofilm formation and defense against phagocytes and imparts tolerance to pH and organic solvents in Proteus vulgaris.

Development of EST-SSR markers related to disease resistance and their application in genetic diversity and evolution analysis in Gossypium.

Genome-wide identification and characterization of the Dof gene family in Medicago truncatula.

Construction of a tissue engineered intervertebral disc with high biological activity using an allogeneic intervertebral disc supplemented with transfected nucleus pulposus cells expressing exogenous dopamine beta-hydroxylase.

Treatment of silicosis with hepatocyte growth factor-modified autologous bone marrow stromal cells: a non-randomized study with follow-up.

Association of polymorphism within the interleukin-28 receptor alpha gene, but not in interleukin-28B, with lower urinary tract symptoms (LUTS) in Chinese.

Molecular genetic analysis of heterosis in interspecific hybrids of Argopecten purpuratus x A. irradians irradians.

Construction of a lentiviral vector encoding heme oxygenase 1 and its introduction into mouse adipose tissue-derived stem cells.

Identification of polymorphisms associated with production traits on chicken (Gallus gallus) chromosome 4.

Distribution and clinal trends of the ABO and Rh genes in select Middle Eastern countries.

Screening of differentially expressed genes in pathological scar tissues using expression microarray.

Expression of c-myc and mutation of the KRAS gene in patients with ovarian mucinous tumors.

Xiayuxue decoction reduces renal injury by promoting macrophage apoptosis in hepatic cirrhotic rats.

Association of vitamin D-binding protein variants with chronic obstructive pulmonary disease: a meta-analysis.

Characteristics of Cyclin B and its potential role in regulating oogenesis in the red claw crayfish (Cherax quadricarinatus).

Effects of intermittent hypobaric hypoxia preconditioning on the expression of neuroglobin and Bcl-2 in the rat hippocampal CA1 area following ischemia-reperfusion.

Arg188His polymorphism in the XRCC2 gene and the risk of ovarian cancer: a meta-analysis.

Sperm chromatin dispersion by formaldehyde in Wistar rats.

Construction and expression of prokaryotic expression vectors fused with genes of Magnaporthe oryzae effector proteins and mCherry.

Performance of peanut mutants and their offspring generated from mixed high-energy particle field radiation and tissue culture.

Diversity of endophytic fungi of Myricaria laxiflora grown under pre- and post-flooding conditions.

Root tip chromosome karyotype analysis of hyacinth cultivars.

Responsivity to PGE2 labor induction involves concomitant differential prostaglandin E receptor gene expression in cervix and myometrium.

Comparison of methods used to identify superior individuals in genomic selection in plant breeding.

Clinical characteristics of men with non-mosaic Klinefelter syndrome in northeastern China: implications for genetic counseling.

Molecular phylogeny and a taxonomic proposal for the genus Streptococcus.

Molecular characterization of interferon regulatory factor 1 in Bubalus bubalis.

Gene expression profile in breast cancer comprising predictive markers for metastatic risk.

Setup errors in cone-beam computed tomography and their effects on acute radiation toxicity in cervical cancer radiotherapy.

Isolation and characterization of novel polymorphic microsatellite markers for Lutjanus erythropterus.

Effect of single nucleotide polymorphisms in the ATP-binding cassette B1 gene on the clinical outcome of traumatic brain injury.

Association between RsaI polymorphism in estrogen receptor ß gene and male infertility.

Identification of copy number variation in the gene for autosomal dominant optic atrophy, OPA1, in a Chinese pedigree.

Effect of flavonoid compounds extracted from Iris species in prevention of carbon tetrachloride-induced liver fibrosis in rats.

Hypertension-mediated enhancement of JNK activation in association with endoplasmic reticulum stress in rat model hippocampus with cerebral ischemia-reperfusion.

COL1A1 gene -1997G/T polymorphism and risk of osteoporosis in postmenopausal women: a meta-analysis.

Effect of age on the immune system and pathology of mice with chronic graft-versus-host disease lupus nephritis.

Correlation of EGFR gene amplification with invasion and metastasis of non-small cell lung cancer.

Gene and protein expression in the oxaliplatin-resistant HT29/L-OHP human colon cancer cell line.

Relationship between serum adiponectin and osteoprotegerin levels and coronary heart disease severity.

Selection of an effective microsatellite marker system for genetic control and analysis of gerbil populations in China.

Metabolic response of LLC xenografted mice to oxythiamine, as measured by [¹H] NMR spectroscopy.

Indirect selection for resistance to ear rot and leaf diseases in maize lines using biplots.

Bone morphogenetic protein 2 and decorin expression in old fracture fragments and surrounding tissues.

Association of MEF2A gene 3'UTR mutations with coronary artery disease.

Identification and functional characterization of esterases in Euschistus heros (Hemiptera, Pentatomidae) and their relationship with thiamethoxam and lambda-cyhalothrin.

Establishment and characterization of a rat model of hyperphosphatemia.

Identification of transgenic cloned dairy goats harboring human lactoferrin and methylation status of the imprinted gene IGF2R in their lungs.

Breaking dormancy in freshly matured seeds of Elymus sibiricus, an important forage grass in the Tibetan Plateau.

Association between SNPs in vascular endothelial growth factor polymorphisms and risk of renal cell carcinoma: a case-control study.

Glutathione S-transferase polymorphisms influence chemotherapy response and treatment outcome in breast cancer.

Associations between single nucleotide polymorphisms and carcass traits in Nellore cattle using high-density panels.

FAS ligand expression in inflammatory infiltrate lymphoid cells as a prognostic marker in oral squamous cell carcinoma.

Cloning, molecular characterization, and expression pattern of FGF5 in Cashmere goat (Capra hircus).

Marker-assisted selection in breeding silkworm strains with high tolerance to fluoride, scaleless wings, and high silk production.

Development and characterization of genic-SSR markers from different Asia lotus (Nelumbo nucifera) types by RNA-seq.

Han Chinese patients with dopa-responsive dystonia exhibit a low frequency of exonic deletion in the GCH1 gene.

Upregulation of fibroblast growth factor 1 in the synovial membranes of patients with late stage osteoarthritis.

Significance of sarcomere gene mutation in patients with dilated cardiomyopathy.

Adaptability and stability of genotypes of sweet sorghum by GGEBiplot and Toler methods.

Δ6-fatty acid desaturase and fatty acid elongase mRNA expression, phagocytic activity and weight-to-length relationships in channel catfish (Ictalurus punctatus) fed alternative diets with soy oil and a probiotic.

Investigation of ERCC1 and ERCC2 gene polymorphisms and response to chemotherapy and overall survival in osteosarcoma.

Identification of single nucleotide polymorphisms of the signal transducer and activator of transcription 3 gene (STAT3) associated with body measurement and carcass quality traits in beef cattle.

Effects of icariin on the expression of ER, VEGF, and KDR in the endometrial cells of thin endometrium.

Pattern of CsICE1 expression under cold or drought treatment and functional verification through analysis of transgenic Arabidopsis.

Backcrossing to increase meiotic stability in triticale.

Genetic divergence of common bean cultivars.

Proliferation and differentiation of human osteoblasts from a type 2 diabetic patient in vitro.

Effect of dehydroepiandrosterone treatment on hormone levels and antioxidant parameters in aged rats.

Endogenous hormonal equilibrium linked to bamboo culm development.

Newly identified gene muscle segment homeobox C may play a role in intermuscular bone development of Hemibarbus labeo.

Cloning and expression pattern analysis of BkGRAS2 from Betula kirghisorum.

Genetic diversity and genetic structure of consecutive breeding generations of golden mandarin fish (Siniperca scherzeri Steindachner) using microsatellite markers.

Changes and clinical significance of serum vaspin levels in patients with type 2 diabetes.

Effect of RAGE polymorphisms on susceptibility to and severity of osteoarthritis in a Han Chinese population: a case-control study.

Expressional analysis of immune-related miRNAs in breast milk.

Identification of single nucleotide polymorphisms in the ASB15 gene and their associations with chicken growth and carcass traits.

Molecular typing of Salmonella from Sergipe, Northeastern Brazil, showing the epidemiological relationship between poultry and human infection.

Effects of glucose and disorders in lipid metabolism on cytokine levels and cognitive impairment in the olanzapine-induced obesity rat model.

Sequence variation in ROP8 gene among Toxoplasma gondii isolates from different hosts and geographical localities.

Evaluation of microsatellite loci from libraries derived from the wild diploid 'Calcutta 4' and 'Ouro' banana cultivars.

Apoptosis induced by lipid-associated membrane proteins from Mycoplasma hyopneumoniae in a porcine lung epithelial cell line with the involvement of caspase 3 and the MAPK pathway.

Suppression of lentivirus-mediated transgenic dendritic cells in graft-versus-host disease after allogeneic bone marrow transplantation in mice.

Resistance of Castanea mollissima Shuhe-WYL strain to Dryocosmus kuriphilus and its molecular mechanism.

A new method of QTL identification for undersaturated maps.

Establishment and rapid detection of a heterozygous missense mutation in the CACNA1F gene by ARMS technique with double-base mismatched primers.

Correlation between polymorphisms in the IL-17A and IL-17F genes and development of coronary artery disease.

Effects of Pax3 and Pax7 expression on muscle mass in the Pekin duck (Anas platyrhynchos domestica).

Protective effects of bifidobacteria on intestines in newborn rats with necrotizing enterocolitis and its regulation on TLR2 and TLR4.

Potential of hypocotyl diameter in family selection aiming at plant architecture improvement of common bean.

Isolation and characterization of novel microsatellite markers from the sika deer (Cervus nippon) genome.

Effect of pulsed electromagnetic field therapy on the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells.

Lack of clinical significance of the ImmuKnow(TM)-Cylex assay for the detection of cellular immune function in patients with renal cell carcinoma.

Analysis of skin color change and related gene expression after crossing of Dongxiang black chicken and ISA layer.

Correlation between single nucleotide polymorphisms in hypoxia-related genes and susceptibility to acute high-altitude pulmonary edema.

Investigation of the value of miR-21 in the diagnosis of early stage HCC and its prognosis: a meta-analysis.

Hypoxia-related gene expression in porcine skeletal muscle tissues at different altitude.

Decreased expression of humanized Fat-1 in porcine fetal fibroblasts following deletion of PGK-neomycin resistance.

Isolation and characterization of yeasts capable of efficient utilization of hemicellulosic hydrolyzate as the carbon source.

Genetic diversity of wild soybean populations in Dongying, China, by simple sequence repeat analysis.

Microvascular remodeling of nasal mucosa in allergic rhinitis induced by an allergen in Sprague-Dawley rats.

Analysis of the association between polymorphisms in the vitamin D receptor (VDR) gene and dental caries in a Chinese population.

Morphological analysis and muscle-associated gene expression during different muscle growth phases of Megalobrama amblycephala.

Genetic variability of genes involved in DNA repair influence treatment outcome in osteosarcoma.

Function Clustering Self-Organization Maps (FCSOMs) for mining differentially expressed genes in Drosophila and its correlation with the growth medium.

Accuracy of enzyme-linked immunospot assay for diagnosis of pleural tuberculosis: a meta-analysis.

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Expression and antibody generation of the cancer-testis antigen, BIOT2-S.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Li, S. Yang, X-F Zhao, J. Cao, M. Wang, J-Y Guo, M-R Wang, M. Fang, J.
Description	Country affiliation: China Author Affiliation: Wang JY ( Key Laboratory of Biological Resources and Ecological Environments of The Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu, China.); Cao M ( Key Laboratory of the Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital, Chengdu, China.); Guo MR ( Key Laboratory of Biological Resources and Ecological Environments of The Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu, China.); Li S ( Key Laboratory of Biological Resources and Ecological Environments of The Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu, China.); Yang XF ( Key Laboratory of Biological Resources and Ecological Environments of The Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu, China.); Wang M ( Key Laboratory of Biological Resources and Ecological Environments of The Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu, China.); Fang J ( Key Laboratory of the Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital, Chengdu, China.); Zhao J ( Key Laboratory of Biological Resources and Ecological Environments of The Chinese Education Ministry, College of Life Science, Sichuan University, Chengdu, China zj804@163.com.)
Abstract	Biot2-S is a mouse cancer-testis antigen gene that was identified using the cross-reactive serological analysis of recombinant cDNA expression libraries (SEREX) technique in the State Key Laboratory of Biotherapy, West China Hospital, Sichuan University. To express BIOT2-S and generate its antibody for further investigation, the Biot2-S prokaryotic recombinant expression vector Biot2-S/pGEX6P-1 was constructed with Escherichia coli DH5 as a cloning vector, and BIOT2-S was expressed in E. coli Rosetta (DE3). The recombinant BIOT2-S was expressed in the form of an inclusion body and the targeted recombinant BIOT2-S was produced at the level of approximately 25% total bacterial proteins after being induced with optimum conditions (0.2 mM isopropyl-ß-D-thiogalactopyranoside for 6 h at 37°C). The target protein was purified by glutathione S-transferase (GST)-trap FF affinity chromatography and detected by western blot. The purified recombinant protein was further confirmed by electrospray ionization quadrupole time-of-flight mass spectrometry after removal of the GST-tags. Then the purified BIOT2-S was used to immunize adult rabbits to generate its antibody. The antibody was purified and its specificity determined. The titer of the antibody was shown to reach 10(4) and the antibody was demonstrated to be able recognize the corresponding protein in the testes of mouse and chicken; the tumor cell lines CT-26 and S180 also reacted with the antibody. This study provides a valuable foundation for further research on the cancer-testis antigen BIOT2-S.
e-ISSN	16765680
Journal	Genetics and Molecular Research
Issue Number	3
Volume Number	14
Language	English
Publisher	Fundação de Pesquisas Científicas de Ribeirão Preto
Publisher Date	2015-07-31
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Antigens, Neoplasm Genetics Immune Sera Chemistry Amino Acid Sequence Animals Biosynthesis Immunology Isolation & Purification Blotting, Western Cell Line, Tumor Chromatography, Affinity Escherichia Coli Mice Molecular Sequence Data Rabbits Recombinant Proteins Solubility Testis Metabolism Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics
Content Type	Text
Resource Type	Article

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