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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Dong, J. Yao, Y. J. Zhao, W. C. Wu, D. Gao, X. |
| Description | Country affiliation: China Author Affiliation: Wu D ( College of Agronomy, Northwest A&F University, Yangling, China.); Dong J ( College of Agronomy, Northwest A&F University, Yangling, China.); Yao YJ ( College of Agronomy, Northwest A&F University, Yangling, China.); Zhao WC ( College of Agronomy, Northwest A&F University, Yangling, China.); Gao X ( College of Agronomy, Northwest A&F University, Yangling, China gx@nwsuaf.edu.cn.) |
| Abstract | The use of appropriate reference genes is essential for the generation of accurate and biologically meaningful results from quantitative real-time PCR (qRT-PCR) analysis. However, studies have found that the expression of most commonly used reference genes is not always independent of the tissues, treatments, or developmental stages studied. geNormPlus, NormFinder, and BestKeeper, were applied and the expression stability of nine candidate genes was evaluated in different data sets during wheat grain development. Varying degrees of diversity in either single or multiple reference genes were observed among the results generated from the different computer programs, parameters, and data sets. Therefore, the reliability of identified reference genes in the flag leaf and the complete set of samples was estimated by monitoring the expression dynamics of three NAM genes (TaNAM-A1, TaNAM-B1, and TaNAM-B2). The results suggest that a single control gene identified by geNormPlus for use with the complete set of samples, and multiple reference genes selected by geNormPlus and NormFinder exclusively for the flag leaf outperformed others owing to the consistent results with previous analyses of these genes, which were normalized against a verified single control gene. Given the limit of NormFinder in gene numbers of multiple reference genes, robust quantification can be achieved by normalizing against Ta27922 or multiple reference genes chosen by geNormPlus for individual tissues. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-09-08 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Gene Expression Regulation, Plant Genes, Essential Genes, Plant Genetics Triticum Cereals Gene Expression Profiling Gene Expression Regulation, Developmental Genetic Variation Real-time Polymerase Chain Reaction Reference Standards Growth & Development Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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