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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Shi, W. S. Tian, Y. Liu, Y. Jin, Y. Jin, H. Z. Manabe, N. |
| Description | Country affiliation: China Author Affiliation: Jin HZ ( Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.); Shi WS ( Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.); Tian Y ( Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.); Liu Y ( Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China.); Jin Y ( Department of Animal Science, College of Agriculture, Yanbian University, Yanji, China yijin@ybu.edu.cn.); Manabe N ( Animal Resource Science Center, University of Tokyo, Kasama, Japan.) |
| Abstract | We determined expression and localization of the anti-apoptotic cellular FLICE inhibitory protein (cFLIP) in the porcine corpora lutea (CL) and corpus albicans (CA) during estrous and pregnancy. The CL and CA were collected at different stages of estrous to determine cFLIP immunolocalization, and mRNA and protein expression. The mRNA expression of the short cFLIP isoform (cFLIPS) was higher at the early and mid CL stages, and lower by the late CL stage (P < 0.01); mRNA expression of the long cFLIP isoform (cFLIPL) was higher at the mid CL stage, and lower at the early and late CL stages (P < 0.01). Levels of cFLIPS and cFLIPL were steady and high during the early and mid CL stages, and had significantly decreased (P < 0.01) by the late stage. The cFLIP protein was highly expressed in the early and mid CL stages of estrous, but weakly ex-pressed in the late stage. Expression of cFLIPS showed no significant difference between preovulatory corpus albicans (CA1) and corpus albicans (CA2) coexistent with the CL from the previous estrus, but cFLIPL mRNA expression was higher during CA1 than CA2. The expression of cFLIPS showed no significant difference between CA1 and CA2, but cFLIPL was not detected. The cFLIP protein was weak-ly expressed in the CA. Expression of cFLIPS and cFLIPL mRNA and proteins was observed in the CL, and the cFLIP protein was highly expressed during pregnancy. We propose that cFLIPS/L acts as a survival factor, and performs an anti-apoptotic function in the porcine CL. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-07-27 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Genetics Casp8 And Fadd-like Apoptosis Regulating Protein Biosynthesis Corpus Luteum Metabolism Estrous Cycle Animals Gene Expression Regulation Pregnancy Protein Isoforms Rna, Messenger Swine Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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