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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Shen, J. Liang, Q. Luo, Y. X. Liu, Z. Yin, L. Ji, T. Shen, F. |
| Description | Country affiliation: China Author Affiliation: Ji T ( Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.); Shen F ( Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.); Liu Z ( Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.); Yin L ( Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.); Shen J ( Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.); Liang Q ( Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.); Luo YX ( Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.) |
| Abstract | The mite (Varroa destructor) has become the greatest threat to apiculture worldwide. As the original host of the mite, Apis cerana can effectively resist the mite. An increased understanding of the resistance mechanisms of Eastern honeybees against V. destructor may help researchers to protect other species against these parasites. In this study, the proteomes of 4 Apis cerana colonies were analyzed using an isobaric tag for relative and absolute quantitation technology. We determined the differences in gene and protein expression between susceptible and resistant colonies that were either unchallenged or challenged by V. destructor. The results showed that a total of 1532 proteins were identified. Gene Ontology enrichment analysis suggested that the transcription factors and basic metabolic and respiratory processes were efficient and feasible factors controlling this resistance, and 12 differentially expressed proteins were identified in Venn analysis. The results were validated by quantitative polymerase chain reaction. This study may provide insight into the genetic mechanisms underlying the resistance of honeybee to mites. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-08-21 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Metabolism Parasitology Insect Proteins Proteomics Varroidae Physiology Animals Electrophoresis, Polyacrylamide Gel Gene Ontology Head Isolation & Purification Real-time Polymerase Chain Reaction Comparative Study Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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