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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Han, J. M. Zhang, Y. Z. Zheng, L. Ya, H. Y. Cheng, Y. W. |
| Description | Country affiliation: China Author Affiliation: Zhang YZ ( Life Science Department, Luoyang Normal University, Luoyang, China.); Cheng YW ( Life Science Department, Luoyang Normal University, Luoyang, China.); Ya HY ( Life Science Department, Luoyang Normal University, Luoyang, China.); Han JM ( Life Science Department, Luoyang Normal University, Luoyang, China.); Zheng L ( Life Science Department, Luoyang Normal University, Luoyang, China.) |
| Abstract | The tree peony leaf is an important vegetative organ that is sensitive to abiotic stress and particularly to high temperature. This sensitivity affects plant growth and restricts tree peony distribution. However, the transcriptomic information currently available on the peony leaf in public databases is limited. In this study, we sequenced the transcriptomes of peony leaves subjected to high temperature using the Illumina HiSeq TM 2000 platform. We performed de novo assembly of 93,714 unigenes (average length of 639.7 bp). By searching the public databases, 22,323 unigenes and 13,107 unigenes showed significant similarities with proteins in the NCBI non-redundant protein database and SWISS-PROT database (E-value < 1e-5), respectively. We assigned 17,340 unigenes to Gene Ontology categories, and we assigned 7618 unigenes to clusters of orthologous groups for eukaryotic complete genomes. By searching the Kyoto Encyclopedia of Genes and Genomes Pathway database, 8014 unigenes were assigned to 6 main categories, including 290 KEGG pathways. To advance research on improving thermotolerance, we identified 24 potential heat shock protein genes with complete open reading frames from the transcriptomic sequences. This is the first study to characterize the leaf transcriptome of tree peony leaf using high-throughput sequencing. The information obtained from the tree peony leaf is valuable for gene discovery, and the identified heat shock protein genes can be used to improve plant stress-tolerance. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-07-28 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Heat-shock Proteins Genetics Paeonia Databases, Nucleic Acid Databases, Protein Gene Expression Profiling High-throughput Nucleotide Sequencing Microsatellite Repeats Molecular Sequence Annotation Temperature Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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