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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Teixeira Florian, E. C. Vidotto, O. Ono, M. A. Rossi, C. Nachi Vidotto, M. C. Pereira, D. A. |
| Description | Country affiliation: Brazil Author Affiliation: Pereira DA ( Programa de Pós Graduação em Ciência Animal, Universidade Estadual de Londrina, Londrina, PR, Brasil.); Teixeira Florian EC ( Laboratório de Protozoologia Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Brasil.); Ono MA ( Laboratório de Imunologia Animal, Departamento de Ciências Patológicas, Universidade Estadual de Londrina, Londrina, PR, Brasil.); Rossi CN ( Laboratório de Protozoologia Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Brasil.) |
| Abstract | Porcine enteropathogenic Escherichia coli (PEPEC) produce an outer membrane protein (intimin) called Paa (porcine attaching and effacing-associated), which is involved in the pathogenesis of E. coli in piglets with diarrhea. The paa gene of a PEPEC strain isolated in Paraná, Brazil, was amplified by polymerase chain reaction, sequenced, and cloned into the pTrcHisTOPO2 vector. The deduced amino acid sequence encoded by the paa gene of PEPEC from Paraná, Brazil, showed 99% homology to the sequences from other PEPEC strains. In this study, the overexpression of recombinant Paa (rPaa) using alternative induction strategies was attempted. The auto-induction protocol showed excellent results for rPaa protein production with 0.4% (w/v) lactose. The rPaa protein is insoluble and was purified with Triton X-100 wash as a total antigen. This method produced a relatively high yield of rPaa. rPaa was recognized by serum from pigs immunized with the PEPEC strain. These results suggest that rPaa could be included in the development of a vaccine against swine colibacillosis. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-07-31 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Enteropathogenic Escherichia Coli Genetics Escherichia Coli Infections Veterinary Escherichia Coli Proteins Animals Cloning, Molecular Isolation & Purification Microbiology Prevention & Control Biosynthesis Gene Expression Sus Scrofa Swine Swine Diseases Transcriptional Activation Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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