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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fan, J. J. Zhang, L. J. Wang, P. Liu, K. Ruan, Y. Y. Cui, Z. H. Xu, X. Zhu, Y. S. |
| Description | Author Affiliation: Fan JJ ( jinjuanf@hotmail.com.); Xu X ( College of Bioscience and Technology, Shenyang Agricultural University, Shenyang, China.); Liu K ( Heilongjiang Academy of Agricultural Sciences, Haerbin, China.); Ruan YY ( College of Bioscience and Technology, Shenyang Agricultural University, Shenyang, China.); Zhu YS ( College of Bioscience and Technology, Shenyang Agricultural University, Shenyang, China.); Cui ZH ( College of Bioscience and Technology, Shenyang Agricultural University, Shenyang, China.) |
| Abstract | Grapevine downy mildew, caused by Plasmopara viticola, is a devastating disease that results in considerable economic losses as well as environmental damage through the repeated application of fungicides. The nucleotide-binding site leucine-rich repeat gene family functions in plant immunoactivity against various pathogens and pests. In this study, the 5' and 3' ends of the resistance gene homology fragment RGA5 were obtained by rapid amplification of cDNA ends. The 4282-base pair full-length cDNA was obtained using gene-specific primers, and the corresponding 1335-amino acid protein sequence contained characteristic nucleotide-binding site leucine-rich repeat domains of plant resistance proteins, including the toll-interleukin receptor type region. Expression of RGA5 during P. viticola infection and abiotic stress was investigated using quantitative real-time polymerase chain reaction. The results showed that treatment with P. viticola and 4 abiotic stimuli (salicyclic acid, methyl-jasmonate, abscisic acid, H2O2) significantly induced RGA5 within 12 days of inoculation. Therefore, RGA5 may play a critical role in protecting grapevines against P. viticola via signaling pathways involving these molecules. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-07-17 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Disease Resistance Genetics Genes, Plant Plant Diseases Microbiology Vitis Amino Acid Sequence Gene Expression Regulation, Plant Molecular Sequence Data Peronospora Physiology Plant Proteins Chemistry Protein Structure, Tertiary Real-time Polymerase Chain Reaction Up-regulation Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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