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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, L. M. Wei, L. Zhang, X. J. Yuan, L. H. Jiang, H. Y. Zhang, Y. Chen, J. P. |
| Description | Country affiliation: China Author Affiliation: Li LM ( Guangdong Entomological Institute, Guangzhou, China.); Wei L ( College of Ecology, Lishui University, Lishui, China.); Jiang HY ( Guangdong Entomological Institute, Guangzhou, China.); Zhang Y ( College of Animal Science of Inner Mongolia Agriculture University, Hohhot, China.); Zhang XJ ( Guangdong Entomological Institute, Guangzhou, China.); Yuan LH ( Guangdong Entomological Institute, Guangzhou, China.); Chen JP ( Guangdong Entomological Institute, Guangzhou, China chenjp@gdei.gd.cn.) |
| Abstract | Anthropogenic activities have seriously impacted wild resources of the Amur sturgeon, Acipenser schrenckii, and more information on local and regional population genetic structure is required to aid the conservation of this species. In this study, we report the development of 12 novel polymorphic microsatellite loci using next-generation sequencing technology, and the genotyping of 24 individuals collected from a sturgeon farm. The results show that the mean number of ob-served alleles per locus is 6.6 (ranging from 2 to 17). Observed and expected heterozygosity values ranged from 0 to 0.958 and from 0.508 to 0.940, respectively. Not a single locus showed significant departure from Hardy-Weinberg equilibrium and no linkage disequilibrium was observed among any pairwise loci. These highly informative microsatellite markers will be useful for genetic diversity and population structure analyses of A. schrenckii and other species of this genus. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-07-14 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Genetics High-throughput Nucleotide Sequencing Microsatellite Repeats Polymorphism, Genetic Animals Molecular Sequence Data Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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