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Effects of isoflurane preconditioning in the delayed phase on myocardial tumor necrosis factor alpha levels and caspase-3 protein expression in a rabbit model of ischemia-reperfusion injury.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ran, Kim Young Zou, Dingquan Xiao, Yayuan Chang, Y. T. Duan, Karen Ou, Yangyong |
| Copyright Year | 2015 |
| Abstract | This study aimed to investigate the protective effects and the mechanisms underlying these effects of isoflurane preconditioning in the delayed phase of myocardial ischemia-reperfusion injury. We randomly divided 30 healthy male New Zealand white rabbits into three groups with 10 rabbits in each group as follows: sham operation group (C group), ischemia-reperfusion group (I/R group), and 2.0% isoflurane preconditioning group (S group). Rabbits in the C group received thoracotomy for 160 min. Rabbits in the I/R group underwent left coronary artery occlusion for 40 min and reperfusion for 120 min. Rabbits in the S group received inhalation of 2.0% isoflurane and 100% oxygen for 2 h; after 24 h, rabbits in this group received the same treatment as that administered to rabbits in the I/R group. We examined the tumor necrosis factor alpha (TNF-α) levels in each group 20 min before occlusion of the left coronary, 20 and 40 min after occlusion of the left coronary artery, and 1 and 2 h after myocardial reperfusion. After reperfusion, immunoblotting was used to measure the myocardial caspase-3 expression levels, and the infarct size was measured using Evans blue and tetrazolium chloride staining. The levels of TNF-α and caspase-3 were lower in the S group than in the I/R group, and the myocardial infarct size decreased in the S group. Thus, isoflurane preconditioning in the delayed phase exerted protective effects by decreasing the myocardial caspase-3 expression and TNF-α production in a rabbit model of ischemia-reperfusion injury. |
| File Format | PDF HTM / HTML |
| DOI | 10.4238/2015.July.3.1 |
| PubMed reference number | 26214404 |
| Journal | Medline |
| Volume Number | 14 |
| Issue Number | 3 |
| Alternate Webpage(s) | http://www.funpecrp.com.br/gmr/year2015/vol14-3/pdf/gmr4927.pdf |
| Alternate Webpage(s) | https://geneticsmr.com/sites/default/files/articles/year2015/vol14-3/pdf/gmr4927_abstract.pdf |
| Alternate Webpage(s) | https://doi.org/10.4238/2015.July.3.1 |
| Journal | Genetics and molecular research : GMR |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
