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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, M. Z. Chen, X. Chen, X. M. Zhang, X. F. Xu, L. L. Wu, S. |
| Description | Country affiliation: China Author Affiliation: Zhang MZ ( Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.); Zhang XF ( Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.); Chen XM ( Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.); Chen X ( Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.); Wu S ( Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.); Xu LL ( Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, China.) |
| Abstract | The enzyme-linked probe hybridization chip utilizes a method based on ligase-hybridizing probe chip technology, with the principle of using thio-primers for protection against enzyme digestion, and using lambda DNA exonuclease to cut multiple PCR products obtained from the sample being tested into single-strand chains for hybridization. The 5'-end amino-labeled probe was fixed onto the aldehyde chip, and hybridized with the single-stranded PCR product, followed by addition of a fluorescent-modified probe that was then enzymatically linked with the adjacent, substrate-bound probe in order to achieve highly specific, parallel, and high-throughput detection. Specificity and sensitivity testing demonstrated that enzyme-linked probe hybridization technology could be applied to the specific detection of eight genetic modification events at the same time, with a sensitivity reaching 0.1% and the achievement of accurate, efficient, and stable results. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-08-10 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Genetic Variation Nucleic Acid Hybridization Reproducibility Of Results Sensitivity And Specificity Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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