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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zheng, W. H. Zhuo, Y. Ding, W. Y. Liang, L. Y. Wang, X. F. Liang, L. |
| Spatial Coverage | China |
| Description | Country affiliation: China Author Affiliation: Zheng WH ( College of Life and Environmental Science, Wenzhou University, Wenzhou, China.); Zhuo Y ( Department of Burn and Plastic Surgery, the 118th Hospital of The People's Liberation Army, Wenzhou, China.); Liang L ( College of Life and Environmental Science, Wenzhou University, Wenzhou, China.); Ding WY ( College of Life and Environmental Science, Wenzhou University, Wenzhou, China.); Liang LY ( College of Life and Environmental Science, Wenzhou University, Wenzhou, China.); Wang XF ( College of Life and Environmental Science, Wenzhou University, Wenzhou, China.) |
| Abstract | Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five populations of C. wenyujin, using ran-dom amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. We found that the percentages of polymorphic loci (PPL) at the species level (98.25% by RAPD and 100% by ISSR) were significantly higher than those at the population level (66.32% by RAPD and 67.14% by ISSR). The highest values of PPL, expected heterozygosity, and Shannon's information index were in Pop1, while the lowest values were in Pop2. Both DNA markers revealed a short genetic distance between Pop1 and Pop2 (0.1424 by RAPD and 0.1904 by ISSR). Phylogenetic trees produced similar results, with Pop1, Pop2, and Pop5 in one group and Pop3 and Pop4 in another. There were no significant correlations between their genetic distances and their geographical distances. The highest genetic diversity was in Pop1 and the lowest was in Pop2, and genetic diversity at the species level was relatively low, but much higher than that at the population level. We recommended the establishment of a germplasm bank, in situ con-servation, and propagation of wild individuals. The present study will improve the evaluation, protection, and utilization of the population resources of C. wenyujin. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-09-08 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Curcuma Genetics Genetics, Population Phylogeny Plant Dispersal Polymorphism, Genetic Conservation Of Natural Resources Classification Genetic Loci Genetic Markers Heterozygote Microsatellite Repeats Phylogeography Plants, Medicinal Random Amplified Polymorphic Dna Technique Seed Bank Organization & Administration Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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