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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Xuan, W. Y. Liu, Z. Q. Feng, D. Luo, M. Y. Zhang, Y. |
| Description | Country affiliation: China Author Affiliation: Xuan WY ( Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China.); Zhang Y ( Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China.); Liu ZQ ( Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China.); Feng D ( Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China.); Luo MY ( Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China.) |
| Abstract | Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. moluccana have various medical and commercial uses. Here, a novel gene coding the biotin carboxyl carrier protein subunit (BCCP) was cloned from A. moluccana L. using the homology cloning method combined with rapid amplification of cDNA end (RACE) technology. The isolated full-length cDNA sequence (designated AM-accB) was 1188 bp, containing a 795-bp open reading frame coding for 265 amino acids. The deduced amino acid sequence of AM-accB contained a biotinylated domain located between amino acids 190 and 263. A. moluccana BCCP shows high identity at the amino acid level to its homologues in other higher plants, such as Vernicia fordii, J. curcas, and Ricinus communis (86, 77, and 70%, respectively), which all contain conserved domains for ACCase activity. The expression of the AM-accB gene during the middle stage of development and maturation in A. moluccana seeds was higher than that in early and later stages. The expression pattern of the AM-accB gene is very similar to that of the oil accumulation rate. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-08-19 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Acetyl-coa Carboxylase Genetics Aleurites Cloning, Molecular Gene Expression Protein Subunits Chemistry Metabolism Amino Acid Sequence Dna, Complementary Fatty Acid Synthase, Type Ii Molecular Sequence Data Plant Oils Sequence Alignment Sequence Analysis, Dna Time Factors Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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