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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhao, W. M. Chang, C. F. Mei, J. X. Zhou, Y. Pan, C. Y. Xu, T. T. Xu, C. S. |
| Description | Country affiliation: China Author Affiliation: Chang CF ( College of Life Science, Henan Normal University, Xinxiang, China.); Zhao WM ( College of Life Science, Henan Normal University, Xinxiang, China.); Mei JX ( College of Life Science, Henan Normal University, Xinxiang, China.); Zhou Y ( College of Life Science, Henan Normal University, Xinxiang, China.); Pan CY ( College of Life Science, Henan Normal University, Xinxiang, China.); Xu TT ( College of Life Science, Henan Normal University, Xinxiang, China.); Xu CS ( College of Life Science, Henan Normal University, Xinxiang, China cellkeylab@126.com.) |
| Abstract | Previous studies have demonstrated that the nuclear factor κB (NF-κB) pathway is involved in promoting cell proliferation. To further explore the regulatory branches and their sequence in the NF-κB pathway in the promotion of hepatocyte proliferation at the transcriptional level during rat liver regeneration, Rat Genome 230 2.0 array was used to detect the expression changes of the isolated hepatocytes. We found that many genes involved in the NF-κB pathway (including 73 known genes and 19 homologous genes) and cell proliferation (including 484 genes and 104 homologous genes) were associated with liver regeneration. Expression profile function (Ep) was used to analyze the biological processes. It was revealed that the NF-κB pathway promoted hepatocyte proliferation through three branches. Several methods of integrated statistics were applied to extract and screen key genes in liver regeneration, and it indicated that eight genes may play a vital role in rat liver regeneration. To confirm the above predicted results, Ccnd1, Jun and Myc were analyzed using qRT-PCR, and the results were generally consistent with that of microarray data. It is concluded that 3 branches and 8 key genes involved in the NF-κB pathway regulate hepatocyte proliferation during rat liver regeneration. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-07-13 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Hepatocytes Cytology Metabolism Liver Regeneration Genetics Nf-kappa B Signal Transduction Animals Cell Proliferation Gene Expression Regulation Models, Biological Oligonucleotide Array Sequence Analysis Rna, Messenger Real-time Polymerase Chain Reaction Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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