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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sa, X. Y. Li, W. Lu, J. Li, C. L. Chen, Z. W. Du, X. Y. Wang, Y. Z. |
| Spatial Coverage | China |
| Description | Country affiliation: China Author Affiliation: Du XY ( Department of Laboratory Animal Science, School of Basic Medical Science, Capital Medical University, Beijing, China.); Li W ( Department of Laboratory Animal Science, School of Basic Medical Science, Capital Medical University, Beijing, China.); Sa XY ( Zhejiang Academy of Medical Science & Zhejiang Center of Laboratory Animals, Hangzhou, China.); Li CL ( Department of Laboratory Animal Science, School of Basic Medical Science, Capital Medical University, Beijing, China.); Lu J ( Department of Laboratory Animal Science, School of Basic Medical Science, Capital Medical University, Beijing, China.); Wang YZ ( Institute of Experimental Animal of Nangfang Hospital, Southern Medical University, Guangzhou, China.); Chen ZW ( Department of Laboratory Animal Science, School of Basic Medical Science, Capital Medical University, Beijing, China.) |
| Abstract | Although gerbils have been widely used in many areas of biological research over many years, there is currently no effective genetic quality control system available. In the present study, we sought to establish a microsatellite marker system for quality control and conducted an optimized analysis of 137 microsatellite loci in two laboratory gerbil populations and one wild population. Independent sample t-tests on the mean effective allele number, mean of Shannon's information index, and mean HE suggested that 28 of the 137 microsatellite markers were informative for gerbil genetic control. Analysis of 4 laboratory gerbil populations and 1 wild population using the 28 microsatellite loci indicated that allele numbers varied from 1.9639 (Guangzhou, GZ) to 6.6071 (North-West wild, NW). The average of HO versus HE was 0.6236/0.3802, 0.6671/0.4159, 0.4185/0.3464, 0.4592/0.3821, and 0.3972/0.4167 for the Beijing, NW, Hangzhou, Dalian, and GZ populations, respectively. The GZ population showed the greatest differentiation, having higher RST and Nei's standard genetic distances. An AMO-VA revealed high genetic differentiation among the five populations (FST = 0.296). The microsatellite system established here is effective and will be important in future studies for genetic quality control and monitoring of gerbil breeds. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-09-21 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Animals, Laboratory Genetics Gerbillinae Animals Genetic Variation Linkage Disequilibrium Microsatellite Repeats Phylogeny Sequence Analysis, Dna Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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