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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ma, Y. Chen, W. X. |
| Description | Country affiliation: China Author Affiliation: Chen WX ( School of Food Science and Technology, Harbin Institute of Technology, Harbin, China.); Ma Y ( School of Food Science and Technology, Harbin Institute of Technology, Harbin, China maying@hit.edu.cn.) |
| Abstract | Calpastatin, an important protein used to regulate meat quality traits in animals, is encoded by the CAST gene. The aim of the present study was to clone the cDNA sequence of the CAST gene and detect the expression of CAST in the tissues of Cyprinus carpio. The cDNA of the C. carpio CAST gene, amplified using rapid amplification of cDNA ends PCR, is 2834 bp in length (accession No. JX275386), contains a 2634-bp open reading frame, and encodes a protein with 877 amino acid residues. The amino acid sequence of the C. carpio CAST gene was 88, 80, and 59% identical to the sequences observed in grass carp, zebrafish, and other fish, respectively. The C. carpio CAST was observed to contain four conserved domains with 54 serine phosphorylation loci, 28 threonine phosphorylation loci, 1 tyrosine phosphorylation loci, and 6 specific protein kinase C phosphorylation loci. The CAST gene showed widespread expression in different tissues of C. carpio. Surprisingly, the relative expression of the CAST transcript in the muscle and heart tissues of C. carpio was significantly higher than in other tissues (P < 0.01). |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-07-03 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Calcium-binding Proteins Biosynthesis Genetics Carps Metabolism Amino Acid Sequence Animals Cloning, Molecular Conserved Sequence Molecular Sequence Data Open Reading Frames Phylogeny Rna, Messenger Real-time Polymerase Chain Reaction Sequence Alignment Sequence Analysis, Dna Transcriptome Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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