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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yu, J. Ru, M. Wang, Y. Liang, Z. S. Peng, L. |
| Description | Country affiliation: China Author Affiliation: Yu J ( College of Life Sciences, Northwest A&F University, Yangling, China.); Wang Y ( The Center of Soil and Water Conservation and Ecoenvironmental Research, Chinese Academy of Sciences, Yangling, China.); Ru M ( The Center of Soil and Water Conservation and Ecoenvironmental Research, Chinese Academy of Sciences, Yangling, China.); Liang Z ( The Center of Soil and Water Conservation and Ecoenvironmental Research, Chinese Academy of Sciences, Yangling, China.) |
| Abstract | Eucommia ulmoides Oliver, the only extant species of Eucommiaceae, is a second-category state-protected endangered plant in China. Evaluation of genetic diversity among some intraspecific hybrid populations of E. ulmoides Oliver is vital for breeding programs and further conservation of this rare species. We studied the genetic diversity of 130 accessions from 13 E. ulmoides intraspecific hybrid populations using inter-simple sequence related (ISSR) and sequence-related amplified polymorphism (SRAP) markers. Of the 100 ISSR primers and 100 SRAP primer combinations screened, eight ISSRs and eight SRAPs were used to evaluate the level of polymorphism and discriminating capacity. A total number of 65 bands were amplified using eight ISSR primers, in which 50 bands (76.9%) were polymorphic, with an average of 8.1 polymorphic fragments per primer. Alternatively, another 244 bands were observed using eight SRAP primer combinations, and 163 (66.8%) of them were polymorphic, with an average of 30.5 polymorphic fragments per primer. The unweighted pair-group method (UPGMA) analysis showed that these 13 populations could be classified into three groups by the ISSR marker and two groups by the SRAP marker. Principal coordinate analysis using SRAP was completely identical to the UPGMA-based clustering, although this was partly confirmed by the results of UPGMA cluster analysis using the ISSR marker. This study provides insights into the genetic background of E. ulmoides intraspecific hybrids. The progenies of the variations 'Huazhong-3', 'big fruit', 'Yanci', and 'smooth bark' present high genetic diversity and offer great potential for E. ulmoides breeding and conservation. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-07-03 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Eucommiaceae Genetics Hybridization, Genetic Cluster Analysis Dna Primers Genetic Markers Genetic Variation Phylogeny Polymorphism, Genetic Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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