NDLI: Genes encoding the biotin carboxylase subunit of acetyl-CoA carboxylase from Brassica napus and parental species: cloning, expression patterns, and evolution

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Year: 2011 Volume: 54 Issue: 12

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Year: 2011 Volume: 54 Issue: 3

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Recovery of a telomere-truncated chromosome via a compensating translocation in maize.

On the bias of recombination fractions, Kosambi's and Haldane's distances based on frequencies of gametes.

Genes encoding the biotin carboxylase subunit of acetyl-CoA carboxylase from Brassica napus and parental species: cloning, expression patterns, and evolution

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Population-specific quantitative trait loci mapping for functional stay-green trait in rice (Oryza sativa L.).

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Year: 2011 Volume: 54 Issue: 2

Year: 2011 Volume: 54 Issue: 1

Genes encoding the biotin carboxylase subunit of acetyl-CoA carboxylase from Brassica napus and parental species: cloning, expression patterns, and evolution

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Li, Zhi-Guo Yin, Wei-Bo Song, Li-Ying Chen, Yu-Hong Guan, Rong-Zhan Wang, Jing-Qiao Wang, Richard R-C Hu, Zan-Min
Description	Country affiliation: China Author Affiliation: Li ZG ( Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.)
Abstract	Comparative genomics is a useful tool to investigate gene and genome evolution. Biotin carboxylase (BC), an important subunit of heteromeric acetyl-CoA carboxylase (ACCase) that is a rate-limiting enzyme in fatty acid biosynthesis in dicots, catalyzes ATP, biotin carboxyl carrier protein, and $CO_{2}$ to form carboxybiotin carboxyl carrier protein. In this study, we cloned four genes encoding BC from Brassica napus L. (namely BnaC.BC.a, BnaC.BC.b, BnaA.BC.a, and BnaA.BC.b), and two were cloned from each of the two parental species Brassica rapa L. (BraA.BC.a and BraA.BC.b) and Brassica oleracea L. (BolC.BC.a and BolC.BC.b). Sequence analyses revealed that in B. napus the genes BnaC.BC.a and BnaC.BC.b were from the C genome of B. oleracea, whereas BnaA.BC.a and BnaA.BC.b were from the A genome of B. rapa. Comparative and cluster analysis indicated that these genes were divided into two major groups, BnaC.BC.a, BnaA.BC.a, BraA.BC.a, and BolC.BC.a in group-1 and BnaC.BC.b, BnaA.BC.b, BraA.BC.b, and BolC.BC.b in group-2. The divergence of group-1 and group-2 genes occurred in their common ancestor 13–17 million years ago (MYA), soon after the divergence of Arabidopsis and Brassica (15–20 MYA). This time of divergence is identical to the previously reported triplicated time of paralogous subgenomes of diploid Brassica species and the divergence date of group-1 and group-2 genes of α-carboxyltransferase, another subunit of heteromeric ACCase, in Brassica. Reverse transcription PCR revealed that the expression level of group-1 and group-2 genes varied in different organs, and the expression patterns of the two groups of genes were similar in different organs, except in flower. However, two paralogs of group-2 BC genes from B. napus could express differently in mature plants tested by generating BnaA.BC.b and BnaC.BC.b promoter–β-glucuronidase (GUS) fusions. The amino acid sequences of proteins encoded by these genes were highly conserved, except the sequence encoding predicted plastid transit peptides. The plastid transit peptides on the BC precursors of Brassica (71–72 amino acid residues) were predicted based on AtBC protein, compared, and confirmed by fusion with green fluorescent protein. Our results will be helpful in elucidating the evolution and the regulation of ACCase in the genus Brassica.
File Format	HTM / HTML
ISSN	08312796
e-ISSN	14803321
Journal	Genome
Issue Number	3
Volume Number	54
Language	English
Publisher	Canadian Science Publishing
Publisher Date	2011-03-01
Publisher Place	Canada
Access Restriction	Open
Subject Keyword	Discipline Genetics Brassica Napus Enzymology Carbon-nitrogen Ligases Genetics Metabolism Evolution, Molecular Genes, Plant Phylogeny Amino Acid Sequence Blotting, Southern Cloning, Molecular Cluster Analysis Computational Biology Gene Components Molecular Sequence Data Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, Dna Sequence Homology Comparative Study Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology Biotechnology

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