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Content Provider | PubMed Central |
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Author | Brothers, Kimberly M. Stella, Nicholas A. Hunt, Kristin M. Romanowski, Eric G. Liu, Xinyu Klarlund, Jes K. Shanks, Robert M. Q. |
Copyright Year | 2015 |
Abstract | The epithelium provides a crucial barrier to infection, and its integrity requiresefficient wound healing. Bacterial cells and secretomes from a subset of testedspecies of bacteria inhibited human and porcine corneal epithelial cell migrationin vitro and ex vivo. Secretomes from 95% of Serratiamarcescens, 71% of Pseudomonas aeruginosa, 29% of Staphylococcusaureus strains, and other bacterial species inhibited epithelial cellmigration. Migration of human foreskin fibroblasts was also inhibited by S.marcescens secretomes indicating that the effect is not cornea specific.Transposon mutagenesis implicated lipopolysaccharide (LPS) core biosynthetic genesas being required to inhibit corneal epithelial cell migration. LPS depletion ofS. marcescens secretomes with polymyxin B agarose rendered secretomesunable to inhibit epithelial cell migration. Purified LPS from S. marcescens,but not from Escherichia coli or S. marcescens strains with mutationsin the waaG and waaC genes, inhibited epithelial cell migration invitro and wound healing ex vivo. Together these data suggest thatS. marcescens LPS is sufficient for inhibition of epithelial woundhealing. This study presents a novel host-pathogen interaction with implications forinfections where bacteria impact wound healing and provides evidence that secretedLPS is a key factor in the inhibitory mechanism. |
Related Links | http://dx.doi.org/10.1038/srep14003 |
Starting Page | 14003 |
File Format | |
ISSN | 20452322 |
e-ISSN | 20452322 |
Journal | Scientific Reports |
Volume Number | 5 |
Language | English |
Publisher | Nature Publishing Group |
Publisher Date | 2015-09-01 |
Access Restriction | Open |
Rights Holder | Nature Publishing Group |
Subject Keyword | Science and technology Research in Higher Education |
Content Type | Text |
Resource Type | Article |
Subject | Multidisciplinary |
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