NDLI: The p53 binding protein PDCD5 is not rate-limiting in DNA damage induced cell death

Content Provider	PubMed Central
Author	Bock, Florian J. Tanzer, Maria C. Haschka, Manuel D. Gerhard, Krumschnabel Sohm, Bénédicte Goetsch, Katrin Kofler, Reinhard Andreas, Villunger
Copyright Year	2015
Abstract	The tumour suppressor p53 is an important mediator of cell cycle arrest and apoptosis in response to DNA damage, acting mainly by transcriptional regulation of specific target genes. The exact details how p53 modulates this decision on a molecular basis is still incompletely understood. One mechanism of regulation is acetylation of p53 on lysine K120 by the histone-acetyltransferase Tip60, resulting in preferential transcription of proapoptotic target genes. PDCD5, a protein with reported pro-apoptotic function, has recently been identified as regulator of Tip60-dependent p53-acetylation. In an effort to clarify the role of PDCD5 upon DNA damage, we generated cell lines in which PDCD5 expression was conditionally ablated by shRNAs and investigated their response to genotoxic stress. Surprisingly, we failed to note a rate-limiting role of PDCD5 in the DNA damage response. PDCD5 was dispensable for DNA damage induced apoptosis and cell cycle arrest and we observed no significant changes in p53 target gene transcription. While we were able to confirm interaction of PDCD5 with p53, we failed to do so for Tip60. Altogether, our results suggest a role of PDCD5 in the regulation of p53 function but unrelated to cell cycle arrest or apoptosis, at least in the cell types investigated.
Related Links	http://dx.doi.org/10.1038/srep11268
Starting Page	11268
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	5
Language	English
Publisher	Nature Publishing Group
Publisher Date	2015-06-01
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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The p53 binding protein PDCD5 is not rate-limiting in DNA damage induced cell death

Interaction between replication protein A and p53 is disrupted after UV damage in a DNA repair-dependent manner.

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Tumor Suppressor P53 Binding Protein 1 (53bp1) Is Involved in DNA Damage–Signaling Pathways

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Modulation of DNA damage-induced apoptosis by cell adhesion is independently mediated by p53 and c-Abl.

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The p53 binding protein PDCD5 is not rate-limiting in DNA damage induced cell death

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The p53 binding protein PDCD5 is not rate-limiting in DNA damage induced cell death

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