NDLI: The C-terminus hot spot region helps in the fibril formation of bacteriophage-associated hyaluronate lyase (HylP2)

Content Provider	PubMed Central
Author	Shukla, Harish Singh, Sudhir Kumar Singh, Amit Kumar Mitra, Kalyan Akhtar, Md. Sohail
Copyright Year	2015
Abstract	The bacteriophage encoded hyaluronate lyases (HylP and HylP2) degrade hyaluronan and other glycosaminoglycans. HylP2 forms a functional fibril under acidic conditions in which its N-terminus is proposed to form the fibrillar core, leading to nucleation and acceleration of fibril formation. Here we report the presence of a hot spot region (A144GVVVY149) towards the carboxy terminus of HylP2, essential for the acceleration of fibril formation. The ‘hot spot’ is observed to be inherently mutated for valines (A178AMVMY183) in case of HylP. The N- terminal swapped chimeras between these phage HLs (NHylP2 CHylP and NHylPCHylP2) or HylP did not form fibrils at acidic pH. However, seeding of prefibrils of HylP2 recompensed nucleation and led to fibrillation in NHylPCHylP2. The V147A mutation in the ‘hot spot’ region abolished fibril formation in HylP2. The M179V and M181V double mutations in the ‘hot spot’ region of HylP led to fibrillation with the seeding of prefibrils. It appears that fibrillation in HylP2 even though is initiated by the N-terminus, is accelerated by the conserved ‘hot spot’ region in the C-terminus. A collagenous (Gly-X-Y)10 motif in the N-terminus and a mutated ‘hot spot’ region in the C-terminus of HylP affect fibrillar nucleation and acceleration respectively.
Related Links	http://dx.doi.org/10.1038/srep14429
Starting Page	14429
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	5
Language	English
Publisher	Nature Publishing Group
Publisher Date	2015-09-23
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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The C-terminus hot spot region helps in the fibril formation of bacteriophage-associated hyaluronate lyase (HylP2)

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The C-terminus hot spot region helps in the fibril formation of bacteriophage-associated hyaluronate lyase (HylP2)

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