NDLI: Delivery of a mucin domain enriched in cysteine residues strengthens the intestinal mucous barrier

Content Provider	PubMed Central
Author	Gouyer, Valérie Laurent, Dubuquoy Catherine, Robbe-masselot Christel, Neut Singer, Elisabeth Plet, Ségolène Geboes, Karel Pierre, Desreumaux Frédéric, Gottrand Desseyn, Jean-luc
Copyright Year	2015
Abstract	A weakening of the gut mucous barrier permits an increase in the access of intestinal luminal contents to the epithelial cells, which will trigger the inflammatory response. In inflammatory bowel diseases, there is an inappropriate and ongoing activation of the immune system, possibly because the intestinal mucus is less protective against the endogenous microflora. General strategies aimed at improving the protection of the intestinal epithelium are still missing. We generated a transgenic mouse that secreted a molecule consisting of 12 consecutive copies of a mucin domain into its intestinal mucus, which is believed to modify the mucus layer by establishing reversible interactions. We showed that the mucus gel was more robust and that mucin O-glycosylation was altered. Notably, the gut epithelium of transgenic mice housed a greater abundance of beneficial Lactobacillus spp. These modifications were associated with a reduced susceptibility of transgenic mice to chemically induced colitis. Furthermore, transgenic mice cleared faster Citrobacter rodentium bacteria which were orally given and mice were more protected against bacterial translocation induced by gavage with adherent–invasive Escherichia coli. Our data show that delivering the mucin CYS domain into the gut lumen strengthens the intestinal mucus blanket which is impaired in inflammatory bowel diseases.
Related Links	http://dx.doi.org/10.1038/srep09577
Starting Page	9577
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	5
Language	English
Publisher	Nature Publishing Group
Publisher Date	2015-05-01
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Delivery of a mucin domain enriched in cysteine residues strengthens the intestinal mucous barrier

Molecular cloning and sequencing of a canine tracheobronchial mucin cDNA containing a cysteine-rich domain.

Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium

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Characterization of the human mucin gene MUC5AC: a consensus cysteine-rich domain for 11p15 mucin genes?

Golgi retention of a trans-Golgi membrane protein, galactosyltransferase, requires cysteine and histidine residues within the membrane-anchoring domain.

Mutations affecting conserved cysteine residues within the extracellular domain of Neu promote receptor dimerization and activation.

Entamoeba histolytica cysteine proteases cleave the MUC2 mucin in its C-terminal domain and dissolve the protective colonic mucus gel

Entamoeba histolytica cysteine proteases cleave the MUC2 mucin in its C-terminal domain and dissolve the protective colonic mucus gel.

Delivery of a mucin domain enriched in cysteine residues strengthens the intestinal mucous barrier

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Delivery of a mucin domain enriched in cysteine residues strengthens the intestinal mucous barrier

Molecular cloning and sequencing of a canine tracheobronchial mucin cDNA containing a cysteine-rich domain.

Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium

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Delivery of a mucin domain enriched in cysteine residues strengthens the intestinal mucous barrier