NDLI: Mechanism of the Antigen-Independent Cytokinergic SPE-7 IgE Activation of Human Mast Cells in Vitro

Content Provider	PubMed Central
Author	Bax, Heather J. Bowen, Holly Dodev, Tihomir S. Sutton, Brian J. Gould, Hannah J.
Copyright Year	2015
Abstract	Release of pro-inflammatory mediators by mast cells is a key feature of allergic disease. The ‘dogma’ is that IgE molecules merely sensitise mast cells by binding FcεRI prior to cross-linking by multivalent allergen, receptor aggregation and mast cell activation. However, certain monoclonal IgE antibodies have been shown to elicit mast cell activation in an antigen-independent cytokinergic manner, and DNP-specific murine SPE-7 IgE is the most highly cytokinergic antibody known. We show that both monovalent hapten and recombinant SPE-7 IgE Fab inhibit its cytokinergic activity as measured by mast cell degranulation and TNF-α release. Using SPE-7 IgE, a non-cytokinergic human IgE and a poorly cytokinergic murine IgE, we reveal that interaction of the Fab region of ‘free’ SPE-7 IgE with the Fab of FcεRI-bound SPE-7 IgE is the basis of its cytokinergic activity. We rule out involvement of IgE Fc, Cε1 and Cλ/κ domains, and propose that ‘free’ SPE-7 IgE binds to FcεRI-bound SPE-7 IgE by an Fv-Fv interaction. Initial formation of a tri-molecular complex (one ‘free’ IgE molecule cross-linking two receptor-bound IgE molecules) leads to capture of further ‘free’ and receptor-bound IgEs to form larger clusters that trigger mast cell activation.
Related Links	http://dx.doi.org/10.1038/srep09538
Starting Page	9538
File Format	PDF
ISSN	20452322
e-ISSN	20452322
Journal	Scientific Reports
Volume Number	5
Language	English
Publisher	Nature Publishing Group
Publisher Date	2015-04-01
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Science and technology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

IgE Trimers Drive SPE-7 Cytokinergic Activity

Cytokinergic IgE Action in Mast Cell Activation

Selective phosphorylation of the IgE receptor in antigen-stimulated rat mast cells.

The PI3-Kinase/mTOR-Targeting Drug NVP-BEZ235 Inhibits Growth and IgE-Dependent Activation of Human Mast Cells and Basophils

Interleukin 3-dependent and -independent mast cells stimulated with IgE and antigen express multiple cytokines

Mast cells modulate transport of CD23/IgE/antigen complex across human intestinal epithelial barrier

Human IgE+ and IgE- are not equivalent to mouse highly cytokinergic IgE.

Induction of tryptase and histamine release from human colon mast cells by IgE dependent or independent mechanisms

Selective phosphorylation of the IgE receptor in antigen-stimulated rat mast cells.

Mechanism of the Antigen-Independent Cytokinergic SPE-7 IgE Activation of Human Mast Cells in Vitro

Similar Documents

IgE Trimers Drive SPE-7 Cytokinergic Activity

Cytokinergic IgE Action in Mast Cell Activation

Selective phosphorylation of the IgE receptor in antigen-stimulated rat mast cells.

The PI3-Kinase/mTOR-Targeting Drug NVP-BEZ235 Inhibits Growth and IgE-Dependent Activation of Human Mast Cells and Basophils

Interleukin 3-dependent and -independent mast cells stimulated with IgE and antigen express multiple cytokines

Mast cells modulate transport of CD23/IgE/antigen complex across human intestinal epithelial barrier

Human IgE+ and IgE- are not equivalent to mouse highly cytokinergic IgE.

Induction of tryptase and histamine release from human colon mast cells by IgE dependent or independent mechanisms

Selective phosphorylation of the IgE receptor in antigen-stimulated rat mast cells.

Mechanism of the Antigen-Independent Cytokinergic SPE-7 IgE Activation of Human Mast Cells in Vitro